Causes of death in newborn C57BL/6J mice
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Abstract
Neonatal mortality in wild-type laboratory mice is an overlooked welfare and financial problem in animal facilities around the world. Causes of death are often not reported and its causes remain unknown.
In this study, 324 newborn pups from two breeding colonies of healthy wildtype C57BL/6 mice underwent post-mortem analysis with special focus on obtaining proof of life after birth, evaluation of stomach contents and observation of congenital abnormalities that could compromise survival.
Based on a combination of lung morphology findings, outcome of lung float test, stomach contents and brown adipose tissue colouration, 21.6% of the pups found dead were considered stillbirths. Of the livebirths, only 3.2% were observed to have milk inside the stomach, indicating successful suckling. Congenital abnormalities were diagnosed only in a small fraction of the pups analysed. These results suggest that starvation was the most common cause of death, followed by stillbirth.
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SciScore for 10.1101/2020.02.25.964551: 5 of 10 (What is this?)
Table 1: Rigor
Institutional Review Board Statement ( Animal Scientific Procedures Act 1986 ) and Decreto-Lei 113/2013 ) and Directive 2010/63/EU of the European Parliament following the 3R᾿s principle of Replacement , Reduction , and Refinement . Randomization not detected. Blinding The pups are born helpless, relatively immobile, blind, hairless, capable of suckling but not able to otherwise fend for themselves. Power Analysis not detected. Sex as a biological variable Wild mice are communal nesters and females often share duties in the care for the young49. Table 2: Resources
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to …
SciScore for 10.1101/2020.02.25.964551: 5 of 10 (What is this?)
Table 1: Rigor
Institutional Review Board Statement ( Animal Scientific Procedures Act 1986 ) and Decreto-Lei 113/2013 ) and Directive 2010/63/EU of the European Parliament following the 3R᾿s principle of Replacement , Reduction , and Refinement . Randomization not detected. Blinding The pups are born helpless, relatively immobile, blind, hairless, capable of suckling but not able to otherwise fend for themselves. Power Analysis not detected. Sex as a biological variable Wild mice are communal nesters and females often share duties in the care for the young49. Table 2: Resources
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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