SARS-CoV-2 Virus Dynamics in Recently Infected People—Data From a Household Transmission Study
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Abstract
We used daily real-time reverse-transcription polymerase chain reaction (RT-PCR) results from 67 cases of SARS-CoV-2 infection in a household transmission study, conducted April 2020–May 2021, to examine the trajectory of cycle threshold (Ct) values, an inverse correlate of viral RNA concentration. Ct values varied across RT-PCR platforms and by participant age. Specimens collected from children and adolescents had higher Ct values and adults aged ≥50 years showed lower Ct values than adults aged 18–49 years. Ct values were lower on days when participants reported experiencing symptoms, with the lowest Ct value occurring 2–6 days after symptom onset.
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SciScore for 10.1101/2022.03.17.22272516: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Wells were seeded with Vero E6-TMPRSS2 cells, to which 100μl of participant specimen was added. Vero E6-TMPRSS2suggested: NoneSoftware and Algorithms Sentences Resources All specimens were tested by RT-PCR using either the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel (EUA CDC-006-00019; with N1 and N2 gene targets and RNase P control; CDC assay) or the ThermoFisher TaqPath™ COVID-19 ComboKit (with S and N gene and ORF1ab targets and MS2 spike control;… SciScore for 10.1101/2022.03.17.22272516: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Wells were seeded with Vero E6-TMPRSS2 cells, to which 100μl of participant specimen was added. Vero E6-TMPRSS2suggested: NoneSoftware and Algorithms Sentences Resources All specimens were tested by RT-PCR using either the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel (EUA CDC-006-00019; with N1 and N2 gene targets and RNase P control; CDC assay) or the ThermoFisher TaqPath™ COVID-19 ComboKit (with S and N gene and ORF1ab targets and MS2 spike control; ThermoFisher assay). ThermoFisher TaqPath™suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Sample size and study period are also limitations, especially in our ability to assess the impact of vaccination status or dissociate vaccination or other demographics from age. Ct values also cannot be converted to a quantitative representation of viral load, and cannot be used to directly infer differences in infectiousness. However, despite these limitations, clinical interpretation of Ct values (or their trajectories) may be “tempting “ (IDSA and AMP joint statement on the use of SARS-CoV-2 PCR cycle threshold (Ct) values for clinical decision-making, page 3) [14]. The present data are directly relevant to these interpretations. Specifically, specimens that were collected within 4 days of symptom onset may represent periods when Ct values are still declining. These findings contribute to our understanding of RT-PCR Ct values during SARS-CoV-2 infections over a broad range of ages, in a community setting with mostly mildly symptomatic illness, and among individuals with a known date of first shedding. While these data were collected prior to Delta and Omicron circulation, and prior to widespread vaccination, they may provide context for interpreting trajectories in Ct values in similar populations during later SARS-CoV-2 outbreaks.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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