No SARS-CoV-2 Neutralization by Intravenous Immunoglobulins Produced From Plasma Collected Before the 2020 Pandemic
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Abstract
The 2020 SARS-CoV-2 pandemic is caused by a zoonotic coronavirus transmitted to humans, similar to earlier events. Whether the other, seasonally circulating coronaviruses induce cross-reactive, potentially even cross-neutralizing, antibodies to the new species in humans is unclear. The question is particularly relevant for people with immune deficiencies, as their health depends on treatment with immunoglobulin preparations that need to contain neutralizing antibodies against the pathogens in their environment. Testing 54 intravenous immunoglobulin preparations, produced from plasma collected in Europe and the United States, confirmed highly potent neutralization of a seasonal coronavirus; however, no cross-neutralization of the new SARS-CoV-2 was seen.
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SciScore for 10.1101/2020.07.30.228213: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Detection of hCoV-229E neutralizing antibodies: The neutralization assay for hCoV-229E antibodies is essentially identical to the SARS-CoV-2, where samples were used undiluted or pre-diluted, then serially diluted in 2-fold steps and mixed 1:2 with 103.0 TCID50/mL hCoV-229E (ATCC, Cat. no. VR-740, Rockville, MD), incubated and titrated on MRC-5 cells (ATCC, Cat. no. CCL-171, Rockville, MD). hCoV-229Esuggested: NoneExperimental Models: Cell Lines Sentences Res… SciScore for 10.1101/2020.07.30.228213: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Detection of hCoV-229E neutralizing antibodies: The neutralization assay for hCoV-229E antibodies is essentially identical to the SARS-CoV-2, where samples were used undiluted or pre-diluted, then serially diluted in 2-fold steps and mixed 1:2 with 103.0 TCID50/mL hCoV-229E (ATCC, Cat. no. VR-740, Rockville, MD), incubated and titrated on MRC-5 cells (ATCC, Cat. no. CCL-171, Rockville, MD). hCoV-229Esuggested: NoneExperimental Models: Cell Lines Sentences Resources Equal volumes of sample dilutions were mixed with virus stock at 103.0 tissue culture infectious doses 50% per milliliter (TCID50/mL) SARS-CoV-2 (strain “BavPat1/2020”, kindly provided by C. Drosten and V. Corman, Charité Berlin, Germany) and incubated for 150min +/-15min, before titration on Vero cells (Cat. no. 84113001, ECACC, Porton Down, Salisbury, UK) in eight-fold replicates per dilution. Verosuggested: NoneDetection of hCoV-229E neutralizing antibodies: The neutralization assay for hCoV-229E antibodies is essentially identical to the SARS-CoV-2, where samples were used undiluted or pre-diluted, then serially diluted in 2-fold steps and mixed 1:2 with 103.0 TCID50/mL hCoV-229E (ATCC, Cat. no. VR-740, Rockville, MD), incubated and titrated on MRC-5 cells (ATCC, Cat. no. CCL-171, Rockville, MD). MRC-5suggested: NoneSoftware and Algorithms Sentences Resources Human plasma pool samples were generated by combination of 6 individual donations obtained in the same calendar week (CW) at Austrian plasma donation centers (BioLife). BioLifesuggested: NoneGraphs and statistical analysis: Graphical illustration and statistical analysis (paired t-tests) were done using GraphPad Prism v8.1.1 software (San Diego, CA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.07.30.228213: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Keywords Primary immunodeficiency; SARS-CoV-2; SARS coronavirus 2 antibody titer; neutralizing antibodies; COVID-19; intravenous immunoglobulin; plasma No SARS-CoV-2 neutralization by IVIGs SARS-COV-2 antibodies are presented. SARS coronavirus 2suggested: NoneDetection of hCoV-229E neutralizing antibodies The neutralization assay for hCoV-229E antibodies is essentially identical to … SciScore for 10.1101/2020.07.30.228213: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Keywords Primary immunodeficiency; SARS-CoV-2; SARS coronavirus 2 antibody titer; neutralizing antibodies; COVID-19; intravenous immunoglobulin; plasma No SARS-CoV-2 neutralization by IVIGs SARS-COV-2 antibodies are presented. SARS coronavirus 2suggested: NoneDetection of hCoV-229E neutralizing antibodies The neutralization assay for hCoV-229E antibodies is essentially identical to the SARS-CoV-2 where samples were used undiluted or pre-diluted, then serially diluted in 2-fold steps and mixe 1:2 with 103.0 TCID50/mL hCoV-229E (ATCC, Cat. no. VR-740, Rockville, MD), incubated an titrated on MRC-5 cells (ATCC, Cat. no. CCL-171, Rockville, MD). hCoV-229Esuggested: (Abcam Cat# ab28169, AB_777848)Thus, a higher infectio rate with hCoV-229E in elder people and consequently the generation of neutralizing hCoV- 229E-specific antibodies in the elder plasma donors would explain the higher hCoV-229E titers found in IVIG lots produced from recovered plasma. 229E-specificsuggested: NoneExperimental Models: Cell Lines Sentences Resources Detection of hCoV-229E neutralizing antibodies The neutralization assay for hCoV-229E antibodies is essentially identical to the SARS-CoV-2 where samples were used undiluted or pre-diluted, then serially diluted in 2-fold steps and mixe 1:2 with 103.0 TCID50/mL hCoV-229E (ATCC, Cat. no. VR-740, Rockville, MD), incubated an titrated on MRC-5 cells (ATCC, Cat. no. CCL-171, Rockville, MD). MRC-5suggested: NoneSoftware and Algorithms Sentences Resources Human plasma pool samples were generated by combination of 6 individual donations obtained in the same calendar week (CW) at Austrian plasma donation centers (BioLife). BioLifesuggested: NoneGraphs and statistical analysis Graphical illustration and statistical analysis (paired t-tests) were done using GraphPad Prism v8.1.1 software (San Diego, CA). GraphPad Prismsuggested: (GraphPad Prism, SCR_002798)Data from additional tools added to each annotation on a weekly basis.
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