Commercial Serology Assays Predict Neutralization Activity against SARS-CoV-2

Article activity feed

1. Version published to 10.1093/clinchem/hvaa262

   Oct 21, 2020
2. 

   ScreenIT

   Jul 12, 2020

   SciScore for 10.1101/2020.07.10.20150946: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The amount of luminescence in HeLa cells that stably expressed the cell surface receptor angiotensin converting enzyme 2 were measured after viral infection.	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   Software and Algorithms
   Sentences	Resources
   The Abbott platform reports results in the form of an Index value (S/C); Index values ≥ 1.4 S/C are considered positive.	Abbott suggested: (Abbott, RRID:SCR_010477)
   Box and whisker …

   SciScore for 10.1101/2020.07.10.20150946: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The amount of luminescence in HeLa cells that stably expressed the cell surface receptor angiotensin converting enzyme 2 were measured after viral infection.	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   Software and Algorithms
   Sentences	Resources
   The Abbott platform reports results in the form of an Index value (S/C); Index values ≥ 1.4 S/C are considered positive.	Abbott suggested: (Abbott, RRID:SCR_010477)
   Box and whisker plots were generated in Rstudio.	Rstudio suggested: (RStudio, RRID:SCR_000432)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   The retrospective analysis of only the 164 seropositive SARS-CoV-2 individuals and not the population of 9530 individuals is a limitation of the study, preventing a fair comparison of the Diazyme platform with the Roche and Abbott SARS-CoV-2 serology platforms. This is because these individuals were effectively pre-screened for SARS-CoV-2 seropositivity before analysis on either the Roche or Abbott platforms. Moreover, out of the 9,530 tests performed, the estimated overall false positive rate of the Diazyme IgG assay was between 0.7 - 0.9%, and is equal to or lower than its published value of 0.9% (13). This suggests that our findings would have been similar if either the Roche total Ig or Abbott IgG assays were used as the screening platform and not the confirmatory platform, as both assays have been reported to have specificity that exceeds 99% (14–16); underlining the dangers of using any one single serology platform to screen low prevalence populations for SARS-CoV-2 serology. We report a comprehensive retrospective study of 164 SARS-CoV-2 seropositive samples from 9,530 SARS-CoV-2 serology tests and for the first time demonstrate that serology results correlate with neutralization. Our study demonstrates the risk of using a single serology platform to identify SARS-CoV-2 seropositive individuals in low prevalence populations and highlights the benefits of a two-platform approach. Finally, the inclusion of serology and neutralization activity found in mild and asymptomat...

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.07.10.20150946v1 on medRxiv

   Jul 11, 2020