Traces of SARS-CoV-2 RNA in Peripheral Blood Cells of Patients with COVID-19

  1. Ahmed Moustafa
  2. Rana Salah Khalel
  3. Ramy K. Aziz

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1089/omi.2021.0068
  2. ScreenIT

    SciScore for 10.1101/2020.05.10.20097055: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Comparison of RNA Abundance and Gene Expression Profiles: Filtered FASTQ sequences were searched with blastx (Altschul et al. 1990) against the RefSeq protein database (O’Leary et al. 2016) (release 99) using DIAMOND (Buchfink, Xie, and Huson 2015) with an e-value cutoff < 1e-10.
    Gene Expression Profiles
    suggested: None
    blastx
    suggested: (BLASTX, RRID:SCR_001653)
    RefSeq
    suggested: (RefSeq, RRID:SCR_003496)
    DIAMOND
    suggested: (DIAMOND, RRID:SCR_009457)
    For functional annotation, the matching RefSeq proteins were annotated by Pfam (Finn et al. 2014) using HMMER (Eddy 2011).
    Pfam
    suggested: (Pfam, RRID:SCR_004726)
    HMMER
    suggested: (Hmmer, RRID:SCR_005305)
    Identified SARS-CoV-2 matching sequences were manually inspected and searched against NCBI “nt” by BLAST (blastn) for verification (Altschul et al. 1990).
    BLAST
    suggested: (BLASTX, RRID:SCR_001653)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.05.10.20097055v2 on medRxiv
  4. Version published to 10.1101/2020.05.10.20097055v1 on medRxiv