Antibody potency, effector function, and combinations in protection and therapy for SARS-CoV-2 infection in vivo

Article activity feed

1. Version published to 10.1084/jem.20201993

   Nov 19, 2020
2. 

   ScreenIT

   Sep 21, 2020

   SciScore for 10.1101/2020.09.15.298067: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Mouse studies and in vivo infections: All mouse studies were performed at the University of North Carolina (Animal Welfare Assurance #A3410-01) using protocols (19-168, 20-114) approved by the UNC Institutional Animal Care and Use Committee (IACUC).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Twelve month old female BALB/c mice (Envigo, #047) were inoculated with the indicated concentration of antibody intraperitoneally 12 hours prior to infection.
   Cell Line Authentication	Contamination: All cell lines have been tested negative for contamination with mycoplasma and parental cell lines were obtained …

   SciScore for 10.1101/2020.09.15.298067: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Mouse studies and in vivo infections: All mouse studies were performed at the University of North Carolina (Animal Welfare Assurance #A3410-01) using protocols (19-168, 20-114) approved by the UNC Institutional Animal Care and Use Committee (IACUC).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Twelve month old female BALB/c mice (Envigo, #047) were inoculated with the indicated concentration of antibody intraperitoneally 12 hours prior to infection.
   Cell Line Authentication	Contamination: All cell lines have been tested negative for contamination with mycoplasma and parental cell lines were obtained from the ATCC.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The half-maximal and 90% inhibitory concentrations (IC50 and IC90) for monoclonal antibodies were determined using 4-parameter nonlinear regression (GraphPad Prism).	IC90 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   For constitutive expression of murine Ace2 (muAce2) in HT1080 cells, a cDNA encoding muAce2 was inserted into a lentiviral vector CSIB 3’ to the SFFV promoter.	HT1080 suggested: CLS Cat# 300216/p517_HT-1080, RRID:CVCL_0317)
   HT1080muAce2 cells were generated by transduction with CSIB-based virus followed by selection with 5 μg/ml blasticidin.	HT1080muAce2 suggested: None
   For virus titration, the caudal lobe of the right lung was homogenized in PBS, resulting homogenate was serial-diluted and inoculated onto confluent monolayers of Vero E6 cells, followed by agarose overlay.	Vero E6 suggested: RRID:CVCL_XD71)
   SARS-CoV-2/SARS-CoV-2 MA pseudotyped reporter virus: SARS-CoV-2 pseudotyped particles were produced by co-transfection of pNL4-3ΔEnv-nanoluc and pSARS-CoV-2-MA-Strunc in 293T cell (Schmidt et al., 2020, Robbiani et al., 2020).	293T suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Twelve month old female BALB/c mice (Envigo, #047) were inoculated with the indicated concentration of antibody intraperitoneally 12 hours prior to infection.	BALB/c suggested: None
   Software and Algorithms
   Sentences	Resources
   The half-maximal and 90% inhibitory concentrations (IC50 and IC90) for monoclonal antibodies were determined using 4-parameter nonlinear regression (GraphPad Prism).	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.09.15.298067 on bioRxiv

   Sep 15, 2020