Identification of a promiscuous conserved CTL epitope within the SARS-CoV-2 spike protein
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SciScore for 10.1101/2021.11.21.469172: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: All animal experiments were approved by the Experimental Animals Committee of SHMC, and all methods were carried out in accordance with relevant guidelines and regulations. Sex as a biological variable Mice: Female BALB/c mice (6-8 weeks of age) were purchased from Beijing Vital Laboratory Animal Technology Co., Ltd. (Beijing, China) and Shanghai Jiesjie Laboratory Animal Co., Ltd. (Shanghai, China), and were kept in SPF conditions. Randomization All animal studies were performed with randomized animal selection. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Mice: Female BALB/c mice (6-8 weeks of age) … SciScore for 10.1101/2021.11.21.469172: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: All animal experiments were approved by the Experimental Animals Committee of SHMC, and all methods were carried out in accordance with relevant guidelines and regulations. Sex as a biological variable Mice: Female BALB/c mice (6-8 weeks of age) were purchased from Beijing Vital Laboratory Animal Technology Co., Ltd. (Beijing, China) and Shanghai Jiesjie Laboratory Animal Co., Ltd. (Shanghai, China), and were kept in SPF conditions. Randomization All animal studies were performed with randomized animal selection. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Mice: Female BALB/c mice (6-8 weeks of age) were purchased from Beijing Vital Laboratory Animal Technology Co., Ltd. (Beijing, China) and Shanghai Jiesjie Laboratory Animal Co., Ltd. (Shanghai, China), and were kept in SPF conditions. BALB/csuggested: NoneRecombinant DNA Sentences Resources Peptide pool derived from SARS-CoV-2 Spike protein: The spike receptor-binding domain (RBD) peptide pool (SARS-CoV-2 spike protein 258-518aa) published previously7 was used for the study (Table 1). Immunization: The mice were injected twice, with a two-week interval, via the intramuscular route (i.m.) with 25 μg pGX9501 expressing a synthetic, optimized sequence of the SARS-CoV-2 full-length spike glycoprotein7 and then electroporation was applied with the Cellectro2000 device. pGX9501suggested: NoneSoftware and Algorithms Sentences Resources Six hours later, the percentage of labeled cells in spleens was detected with LSRFortessa flow cytometry (BD) and analyzed by FlowJo (TreeStar). FlowJosuggested: (FlowJo, RRID:SCR_008520)Statistics were performed using GraphPad Prism 7 software. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 23. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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