SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species

  1. Mehdi Moustaqil
  2. Emma Ollivier
  3. Hsin-Ping Chiu
  4. Sarah Van Tol
  5. Paulina Rudolffi-Soto
  6. Christian Stevens
  7. Akshay Bhumkar
  8. Dominic J. B. Hunter
  9. Alexander N. Freiberg
  10. David Jacques
  11. Benhur Lee
  12. Emma Sierecki
  13. Yann Gambin

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Abstract

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  1. Version published to 10.1080/22221751.2020.1870414
  2. ScreenIT

    SciScore for 10.1101/2020.06.05.135699: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    The following commercial antibodies were used: anti-IRF3 (ab68481) and anti-ACE2 (ab108252) from Abcam, anti-NLRP12 (PA5-21027) from Invitrogen, anti-TAB1 (#3226) from Cell Signaling, anti-SARS-CoV-2 N (GTX632269) from Genetex, anti- TMPRSS2 (sc-515727) from Santa Cruz Biotechnology, anti-COXIV (11242-1-AP) from Proteintech.
    anti-IRF3
    suggested: (Abcam Cat# ab68481, RRID:AB_11155653)
    anti-ACE2
    suggested: (Abcam Cat# ab108252, RRID:AB_10864415)
    anti-NLRP12
    suggested: (Thermo Fisher Scientific Cat# PA5-21027, RRID:AB_11152858)
    anti-TAB1
    suggested: (Cell Signaling Technology Cat# 3226, RRID:AB_2140247)
    anti-SARS-CoV-2 N (GTX632269
    suggested: None
    anti- TMPRSS2
    suggested: (Santa Cruz Biotechnology Cat# sc-33533, RRID:AB_2205595)
    anti-COXIV
    suggested: (Proteintech Cat# 11242-1-AP, RRID:AB_2085278)
    Recombinant DNA
    SentencesResources
    Alexandrov and sourced from Addgene (Addgene plasmid # 67137; http://n2t.net/addgene:67137; RRID:Addgene_67137).
    detected: RRID:Addgene_67137)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 11, 12 and 13. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • No funding statement was detected.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.06.05.135699v1 on bioRxiv