A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners
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SciScore for 10.1101/2020.10.31.20220608: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The animal study was reviewed and approved by the Institutional Animal Care and Use Committee of Universidad de Chile, under protocol number 20370–VET–UCH, Biosafety Committee of Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile under protocol number 161 and the human study was reviewed and approved under protocol 16-066 Scientific Ethical Committee on Health Sciences of Pontifical Universidad Católica de Chile. Randomization Sequences were aligned to a set of reference SARS-CoV-2 sequences including all Chilean sequences submitted to GISAID(20) (n=167), sequences with highest similarity deposited on GenBank identified using BLAST(21) … SciScore for 10.1101/2020.10.31.20220608: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The animal study was reviewed and approved by the Institutional Animal Care and Use Committee of Universidad de Chile, under protocol number 20370–VET–UCH, Biosafety Committee of Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile under protocol number 161 and the human study was reviewed and approved under protocol 16-066 Scientific Ethical Committee on Health Sciences of Pontifical Universidad Católica de Chile. Randomization Sequences were aligned to a set of reference SARS-CoV-2 sequences including all Chilean sequences submitted to GISAID(20) (n=167), sequences with highest similarity deposited on GenBank identified using BLAST(21) (n=337), and a total of 1000 sequences randomly selected from the GISAID repository using FastaUtils package in R(22). Blinding Cell cultures with no CPE were frozen, thawed, and subjected to three blind passages with inoculation of fresh Vero E6 cell cultures with the lysates as described above. Power Analysis not detected. Sex as a biological variable On May 2020 a COVID-19 diagnosis was confirmed by SARS-CoV-2 rtRT-PCR for the humans in the studied household – a male of around 30 years old and a female of around 60 years old. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Serological testing: Serum samples were tested via a commercial ELISA test for SARS-CoV-2 antibody detection, the ID SCREEN® SARS-COV-2 Double Antigen Multi Species(18) (IDVET, Grabels, FRANCE). SARS-CoV-2suggested: NoneExperimental Models: Cell Lines Sentences Resources Cell cultures with no CPE were frozen, thawed, and subjected to three blind passages with inoculation of fresh Vero E6 cell cultures with the lysates as described above. Vero E6suggested: RRID:CVCL_XD71)Software and Algorithms Sentences Resources Sequences were aligned to a set of reference SARS-CoV-2 sequences including all Chilean sequences submitted to GISAID(20) (n=167), sequences with highest similarity deposited on GenBank identified using BLAST(21) (n=337), and a total of 1000 sequences randomly selected from the GISAID repository using FastaUtils package in R(22). FastaUtilssuggested: NoneThe sequences were aligned using MAFFT v7.402 using CIPRESS computational resources(23). MAFFTsuggested: (MAFFT, RRID:SCR_011811)The aligned sequences were visualized in AliView v1.26(24). AliViewsuggested: (AliView, RRID:SCR_002780)The phylogenetic tree was visualized using Figtree. Figtreesuggested: (FigTree, RRID:SCR_008515)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:The viral RNA in samples may indirectly denote SARS-CoV-2 viral excretion and can serve, with limitations, as a proxy for active viral replication. Indeed, SARS- CoV-2 antibodies detected by ELISA confirmed the infection in cat 2. Our household study thus provides preliminary guidance for managing the care and quarantine of cats in households in which SARS-CoV-2 is present. The results, particularly the similarity of genome sequences depicted in the phylogenetic analysis, strongly support the idea that SARS-CoV-2 can be transmitted between humans and cats living in the same household. It is suspected that the humans were infected following exposure to SARS-CoV-2-positive neighbors days before. We think it likely that cat 1 was the first infected of the three cats because this animal had closer contact than the other two cats with human 1 (male), sharing his bed. This is supported by our observation that viral RNA from human 1 and the cats had identical sequences whereas the female human’s viral RNA differed by two polymorphisms. Transmission between humans and cats in the same household accords with the literature on viral infections in animals. Several animal species are permissive for virus infection and replication in the upper respiratory tract(1) and it has been suggested that the species barrier of SARS-CoV-2 might be weak since the ACE2 host receptor may allow virus attachment even after some amino acid changes(28). SARS-CoV-2 can be transmitted to animals by direct or...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
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- No protocol registration statement was detected.
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