Zinc pyrithione is a potent inhibitor of PL Pro and cathepsin L enzymes with ex vivo inhibition of SARS-CoV-2 entry and replication
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2022.03.03.482819: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources 68 Recombinant PLPro was prepared according to the modified protocol.69 Briefly, the sequence was codon optimized, synthesized and cloned into pET28b/32 (pET28b with ampicillin resistance) using AcoI and XhoI sites. pET28b/32suggested: NoneSoftware and Algorithms Sentences Resources Data were analysed and visualized in GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data …
SciScore for 10.1101/2022.03.03.482819: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Recombinant DNA Sentences Resources 68 Recombinant PLPro was prepared according to the modified protocol.69 Briefly, the sequence was codon optimized, synthesized and cloned into pET28b/32 (pET28b with ampicillin resistance) using AcoI and XhoI sites. pET28b/32suggested: NoneSoftware and Algorithms Sentences Resources Data were analysed and visualized in GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:However, the results are not clinically applicable due to various limitations of the study.49 On the other hand, an ongoing clinical trial of intravenous administration of ZnCl2 has so far confirmed its safe use and shown an increase in serum zinc levels.50,51 However, oral administration of the drug is much more patient-friendly and is preferred over intravenous use. Ionophores, molecules that enable the transport of metal ions across a lipid cell membrane, increase zinc uptake and facilitate zinc absorption, thus combating COVID-19.13,52–54 In a multi-center study testing zinc sulphate and hydroxychloroquine as potential treatments, increased hospital discharge and decreased in-hospital mortality were observed.55 However, hydroxychloroquine as a therapeutic against COVID-19 should be considered with great caution as it may significantly increase the risk of cardiac arrhythmias. Similarly, a high risk of cardiac dysfunction has been reported for chloroquine.56–58 Therefore, the choice of an appropriate chelating agent for zinc is of utmost importance. Pyrithione a is a known ionophore with the ability to bind zinc ions, increase their intracellular concentration59 and as such has recognized antiviral activity against Coronavirus (SARS-CoV), Arterivirus, Coxsackievirus, Mengovirus, Picornavirus and Rhinovirus.60 A decade ago, te Velthius et al. demonstrated that the combination of Zn2+ ions and pyrithione a effectively inhibited the replication of SARS-CoV via Zn2+ suppressio...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-