Structural and functional conservation of the programmed −1 ribosomal frameshift signal of SARS coronavirus 2 (SARS-CoV-2)

Article activity feed

1. Version published to 10.1074/jbc.ac120.013449

   Jul 1, 2020
2. 

   Version published to 10.1101/2020.03.13.991083v2 on bioRxiv

   Jun 15, 2020
3. 

   ScreenIT

   Jun 5, 2020

   SciScore for 10.1101/2020.03.13.991083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Cell culture and plasmid transfection: Human embryonic kidney (HEK293T/17) (CRL-11268) and HeLa (CCL-2) cells were purchased from the American Type Culture Collection (Manassas, VA)	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   HEK293T and HeLa cells were seeded at 4 × 104 cells per well into 24-well plates.	HEK293T suggested: None
   Software and Algorithms
   Sentences	Resources
   The EMBOSS Water pairwise alignment tool was used to …

   SciScore for 10.1101/2020.03.13.991083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Cell culture and plasmid transfection: Human embryonic kidney (HEK293T/17) (CRL-11268) and HeLa (CCL-2) cells were purchased from the American Type Culture Collection (Manassas, VA)	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)
   HEK293T and HeLa cells were seeded at 4 × 104 cells per well into 24-well plates.	HEK293T suggested: None
   Software and Algorithms
   Sentences	Resources
   The EMBOSS Water pairwise alignment tool was used to identify sequences in the SARS-CoV-2 genome most similar to the SARS-CoV −1 PRF sequence.	EMBOSS suggested: (EMBOSS, RRID:SCR_008493)
   Statistical analyses were conducted using Student’s t test or one-way analysis of variance as appropriate using Prism 8 software (GraphPad).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
4. 

   Version published to 10.1101/2020.03.13.991083v1 on bioRxiv

   Mar 15, 2020