Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody

  1. Jonathan L. Torres
  2. Gabriel Ozorowski
  3. Emanuele Andreano
  4. Hejun Liu
  5. Jeffrey Copps
  6. Giulia Piccini
  7. Lorena Donnici
  8. Matteo Conti
  9. Cyril Planchais
  10. Delphine Planas
  11. Noemi Manganaro
  12. Elisa Pantano
  13. Ida Paciello
  14. Piero Pileri
  15. Timothée Bruel
  16. Emanuele Montomoli
  17. Hugo Mouquet
  18. Olivier Schwartz
  19. Claudia Sala
  20. Raffaele De Francesco
  21. Ian A. Wilson
  22. Rino Rappuoli
  23. Andrew B. Ward

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Abstract

Clinical candidate monoclonal antibody J08 binds the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S-protein independent of known escape mutations and is able to potently neutralize most variants of concern (VoCs). Here, we explore these properties using cell-based assays and structural studies. A relatively small epitope footprint high on the receptor binding domain (RBD) ridge and the ability to bind multiple conformational states of the S-protein contribute to strong neutralization across several variants.

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  1. Version published to 10.1073/pnas.2120976119
  2. Version published to 10.1101/2021.09.28.462234v2 on bioRxiv
  3. ScreenIT

    SciScore for 10.1101/2021.09.28.462234: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    No key resources detected.

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

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  4. Version published to 10.1101/2021.09.28.462234v1 on bioRxiv