Structure of SARS-CoV-2 ORF8, a rapidly evolving immune evasion protein
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Abstract
The structure of the SARS-CoV-2 ORF8 protein reveals two novel intermolecular interfaces layered onto an ORF7 fold. One is mediated by a disulfide bond, the other is noncovalent, and both are novel with respect to SARS-CoV. The structural analysis here establishes a molecular framework for understanding the rapid evolution of ORF8, its contributions to COVID-19 pathogenesis, and the potential for its neutralization by antibodies.
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SciScore for 10.1101/2020.08.27.270637: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Integrated reflections were scaled, merged and truncated using the CCP4 software suit (20) CCP4suggested: (CCP4, RRID:SCR_007255)Initial phases were obtained for the SeMet dataset using the Phenix autosol pipeline (21). Phenixsuggested: (Phenix, RRID:SCR_014224)Iterative rounds of manual model building and refinement were carried out using Coot (23) and Phenix Refine (21) respectively. Cootsuggested: (Coot, RRID:SCR_014222)Structural figures were produced using the program PyMOL … SciScore for 10.1101/2020.08.27.270637: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Integrated reflections were scaled, merged and truncated using the CCP4 software suit (20) CCP4suggested: (CCP4, RRID:SCR_007255)Initial phases were obtained for the SeMet dataset using the Phenix autosol pipeline (21). Phenixsuggested: (Phenix, RRID:SCR_014224)Iterative rounds of manual model building and refinement were carried out using Coot (23) and Phenix Refine (21) respectively. Cootsuggested: (Coot, RRID:SCR_014222)Structural figures were produced using the program PyMOL (https://pymol.org). PyMOLsuggested: (PyMOL, RRID:SCR_000305)Primary sequence alignment of ORF8 homologs was performed using the ClustalOmega server (24). ClustalOmegasuggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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