Prolonged and extended impacts of SARS-CoV-2 on the olfactory neurocircuit

Article activity feed

1. Version published to 10.1038/s41598-022-09731-7

   Apr 6, 2022
2. 

   ScreenIT

   Nov 19, 2021

   SciScore for 10.1101/2021.11.04.467274: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: All animal studies are reviewed and approved by the Institutional Animal Care and Use Committee at UTMB and are conducted according to the National Institutes of Health guidelines. Euthanasia Agents: Immunohistochemistry: Hamsters were euthanized using a high-flow rate of CO2 followed by thoracotomy at each time point to collect samples.
   Sex as a biological variable	Animal experiments: Six-week-old female Syrian golden hamsters were purchased from Charles River.
   Randomization	Three regions were randomly selected, with the regions being at least 100 μm apart, and the averaged values were used.
   Blinding	Statistical analysis: To prevent arbitrary analysis, all data were randomly and blindly …

   SciScore for 10.1101/2021.11.04.467274: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: All animal studies are reviewed and approved by the Institutional Animal Care and Use Committee at UTMB and are conducted according to the National Institutes of Health guidelines. Euthanasia Agents: Immunohistochemistry: Hamsters were euthanized using a high-flow rate of CO2 followed by thoracotomy at each time point to collect samples.
   Sex as a biological variable	Animal experiments: Six-week-old female Syrian golden hamsters were purchased from Charles River.
   Randomization	Three regions were randomly selected, with the regions being at least 100 μm apart, and the averaged values were used.
   Blinding	Statistical analysis: To prevent arbitrary analysis, all data were randomly and blindly analyzed by two trained human operators.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Samples were incubated for 12 hr in a solution containing the following primary antibodies: olfactory marker protein (OMP, goat polyclonal, 1:5000 dilution; Wako Chemicals), SARS-CoV-2 Nucleocapsid antibody (rabbit polyclonal, 1:100; Sino Biological), anti-Iba1 (rabbit polyclonal, 1:300; Wako), anti-Iba1 for paraffin section (rabbit polyclonal, 1:300; Wako), anti-Iba1 (goat polyclonal, 1:300; Abcam), anti-NQO1 (rabbit polyclonal, 1:300; Cell Signaling), anti-NQO1 (goat polyclonal, 1:300; Abcam), and anti-GFAP antibody (mouse monoclonal, 1:500; Millipore).	SARS-CoV-2 suggested: (Bio X Cell Cat# BE0359, RRID:AB_2894778) anti-Iba1 suggested: None anti-NQO1 suggested: None anti-GFAP suggested: None
   Primary antibodies were detected by incubation for 1 hr with a solution containing the following secondary antibodies; goat anti-rabbit Fluor 488, goat anti-mouse Fluor 568, donkey anti-goat Alexa Fluor 488, and donkey anti-rabbit Alexa Fluor 568 (1:200; Invitrogen).	anti-rabbit suggested: (Molecular Probes Cat# A-11036, RRID:AB_10563566) anti-mouse Fluor 568, donkey anti-goat Alexa Fluor 488, and donkey anti-rabbit Alexa Fluor 568 suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   SARS-CoV-2 (USA/WA-1/2020) was propagated in Vero E6 cells with DMEM supplemented with 2% FBS.	Vero E6 suggested: RRID:CVCL_XD71)
   Software and Algorithms
   Sentences	Resources
   Subsequently, binary images were overlapped onto the original images and the densities were automatically measured using ImageJ software.	ImageJ suggested: (ImageJ, RRID:SCR_003070)
   R (https://www.r-project.org/) and custom-written Python scripts were used for statistical analysis.	https://www.r-project.org/ suggested: (R Project for Statistical Computing, RRID:SCR_001905) Python suggested: (IPython, RRID:SCR_001658)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 31. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.11.04.467274v1 on bioRxiv

   Nov 5, 2021