Sex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets

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1. Version published to 10.1038/s41598-021-93855-9

   Jul 15, 2021
2. 

   ScreenIT

   Jan 13, 2021

   SciScore for 10.1101/2021.01.12.426381: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Ethics Statement: All work was conducted in accordance with the Canadian Council of Animal Care (CCAC) guidelines, AUP number 20200016 by the University Animal Care Committee (
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animals, Infections, and Tissue Collection: Adult female (1 year), adult male (1 year) and aged male (2 years) ferrets with intact sex organs were purchased from Triple F Farms (Gillett, PA, USA).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus was diluted to 100 TCID50 in vDMEM and used at a 1:1 ratio to plasma, incubated …

   SciScore for 10.1101/2021.01.12.426381: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Ethics Statement: All work was conducted in accordance with the Canadian Council of Animal Care (CCAC) guidelines, AUP number 20200016 by the University Animal Care Committee (
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animals, Infections, and Tissue Collection: Adult female (1 year), adult male (1 year) and aged male (2 years) ferrets with intact sex organs were purchased from Triple F Farms (Gillett, PA, USA).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus was diluted to 100 TCID50 in vDMEM and used at a 1:1 ratio to plasma, incubated at 37°C for 1 h and added to cultured Vero-76 cells in 96 well plates for 1 h at 37°C.	Vero-76 suggested: None
   Software and Algorithms
   Sentences	Resources
   Trimmed reads were aligned (HISAT277) to the Mustela putorius furo NCBI reference genome assembly version MusPutFur1.0 and sorted using SAMtools.	SAMtools suggested: (SAMTOOLS, RRID:SCR_002105)
   Reads were counted and assigned to gene meta-features using the program featureCounts 78 (Subread package).	featureCounts suggested: (featureCounts, RRID:SCR_012919)
   Sequence and gene expression data are available at the Gene Expression Omnibus (	Gene Expression Omnibus suggested: (Gene Expression Omnibus (GEO, RRID:SCR_005012)
   Statistical Analysis: Unpaired, unequal variance, two-tail Student’s t-test or one-way ANOVAs were conducted using GraphPad Prism8 (San Diego, USA).	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: Thank you for sharing your data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2021.01.12.426381v1 on bioRxiv

   Jan 12, 2021