Functional profiling of COVID-19 respiratory tract microbiomes
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Abstract
In response to the ongoing global pandemic, characterizing the molecular-level host interactions of the new coronavirus SARS-CoV-2 responsible for COVID-19 has been at the center of unprecedented scientific focus. However, when the virus enters the body it also interacts with the micro-organisms already inhabiting the host. Understanding the virus-host-microbiome interactions can yield additional insights into the biological processes perturbed by viral invasion. Alterations in the gut microbiome species and metabolites have been noted during respiratory viral infections, possibly impacting the lungs via gut-lung microbiome crosstalk. To better characterize microbial functions in the lower respiratory tract during COVID-19 infection, we carry out a functional analysis of previously published metatranscriptome sequencing data of bronchoalveolar lavage fluid from eight COVID-19 cases, twenty-five community-acquired pneumonia patients, and twenty healthy controls. The functional profiles resulting from comparing the sequences against annotated microbial protein domains clearly separate the cohorts. By examining the associated metabolic pathways, distinguishing functional signatures in COVID-19 respiratory tract microbiomes are identified, including decreased potential for lipid metabolism and glycan biosynthesis and metabolism pathways, and increased potential for carbohydrate metabolism pathways. The results include overlap between previous studies on COVID-19 microbiomes, including decrease in the glycosaminoglycan degradation pathway and increase in carbohydrate metabolism. The results also suggest novel connections to consider, possibly specific to the lower respiratory tract microbiome, calling for further research on microbial functions and host-microbiome interactions during SARS-CoV-2 infection.
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SciScore for 10.1101/2020.05.01.073171: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources A PRROMenade14 database search index was constructed using the KEGG hierarchy and a total of 21.2M bacterial and 53k viral annotated protein domain sequences (of minimum length 5 AA), obtained on June 6, 2020 from the IBM Functional Genomics Platform15 (previously known as OMXWare). KEGGsuggested: (KEGG, RRID:SCR_012773)Multidimensional scaling (Matlab function cmdscale, p = 2) and permutational multivariate analysis of variance (f_permanova, iter = 100, 000, from the Fathom toolbox28 for Matlab) were applied on pairwise Spearman’s distances (Fig. 1B). Matlabsuggested: (MATLAB, RRID:SCR_0016…SciScore for 10.1101/2020.05.01.073171: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources A PRROMenade14 database search index was constructed using the KEGG hierarchy and a total of 21.2M bacterial and 53k viral annotated protein domain sequences (of minimum length 5 AA), obtained on June 6, 2020 from the IBM Functional Genomics Platform15 (previously known as OMXWare). KEGGsuggested: (KEGG, RRID:SCR_012773)Multidimensional scaling (Matlab function cmdscale, p = 2) and permutational multivariate analysis of variance (f_permanova, iter = 100, 000, from the Fathom toolbox28 for Matlab) were applied on pairwise Spearman’s distances (Fig. 1B). Matlabsuggested: (MATLAB, RRID:SCR_001622)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations of the study include small sample size and lack of clinical data, hence possible connections between administered therapies and the microbiome remain elusive. The findings from this analysis call for further in-depth research on microbial functions and host-microbiome interactions during SARS-CoV-2 infection. Examining metatranscriptome sequencing reads with this comparative functional annotation framework could yield additional insights into microbiome alterations also in other diseases.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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