Aerosol and surface contamination of SARS-CoV-2 observed in quarantine and isolation care
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Abstract
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) originated in Wuhan, China in late 2019, and its resulting coronavirus disease, COVID-19, was declared a pandemic by the World Health Organization on March 11, 2020. The rapid global spread of COVID-19 represents perhaps the most significant public health emergency in a century. As the pandemic progressed, a continued paucity of evidence on routes of SARS-CoV-2 transmission has resulted in shifting infection prevention and control guidelines between classically-defined airborne and droplet precautions. During the initial isolation of 13 individuals with COVID-19 at the University of Nebraska Medical Center, we collected air and surface samples to examine viral shedding from isolated individuals. We detected viral contamination among all samples, supporting the use of airborne isolation precautions when caring for COVID-19 patients.
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SciScore for 10.1101/2020.03.23.20039446: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend …
SciScore for 10.1101/2020.03.23.20039446: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.03.23.20039446: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources *E gene target primers and probe: Probe: 5’/56-FAM/ACACTAAGCC/ZEN /ATCCTTACTGCGCTTCG/3AIBk FG/-3’ Primer 1: 5’-ATATTGCAGCAGTACGCACAC A-3' Primer 2: 5’-ACAGGTACGTTAATAGTTAAT AGCGT-3' ssDNA E Target Sequence: 5’TTCGGAAGAGACAGGTACGTTA ATAGTTAATAGCGTACTTCTTTTT CTTGCTTTCGTG GTATTCTTGCTAGTTACACTAGCC ATCCTTACTGCGCTTCGATTGTGT GCGTACTGCTGC AATATTGTTAACGTG-3’ ssDNA N Target Sequence: 5’ACCAAAAGATCACATTGGCACC … SciScore for 10.1101/2020.03.23.20039446: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources *E gene target primers and probe: Probe: 5’/56-FAM/ACACTAAGCC/ZEN /ATCCTTACTGCGCTTCG/3AIBk FG/-3’ Primer 1: 5’-ATATTGCAGCAGTACGCACAC A-3' Primer 2: 5’-ACAGGTACGTTAATAGTTAAT AGCGT-3' ssDNA E Target Sequence: 5’TTCGGAAGAGACAGGTACGTTA ATAGTTAATAGCGTACTTCTTTTT CTTGCTTTCGTG GTATTCTTGCTAGTTACACTAGCC ATCCTTACTGCGCTTCGATTGTGT GCGTACTGCTGC AATATTGTTAACGTG-3’ ssDNA N Target Sequence: 5’ACCAAAAGATCACATTGGCACC CGCAATCCTGCTAACAATGCTGCA ATCGTGCTACA ACTTCCTCAAGGAACAACATTGCC AAAAGGCTTCTACGCAGAAGGGAG CAGAGGCGG CAGTCAAGCCTCTTCTCGTTCCTC ATCACGTAGT-3’ Cell Culture Assays Vero E6 cells were used to culture virus from environmental samples. Vero E6suggested: CVCL_XD71Software and Algorithms Sentences Resources The Primer-BLAST 21 tool was used to examine the specificity of the assay beyond what was described in the original publication. Primer-BLASTsuggested: (Primer-BLAST, SCR_003095)Results from Barzooka: We also found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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