SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation
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SciScore for 10.1101/2020.07.07.191676: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Fractions were precipitated with trichloroacetic acid (TCA) and subjected to Western blot analysis (anti-His antibody, Clontech). anti-Hissuggested: NoneExperimental Models: Cell Lines Sentences Resources Preparation of viral 5’-UTR mRNA and reporter RLuc mRNA: Preparation of HeLa translation-competent lysates: HeLa S3 lysates were prepared similarly as described before15. HeLasuggested: NoneFor this, frozen HEK293E cells were thawed and resuspended in 2x … SciScore for 10.1101/2020.07.07.191676: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Fractions were precipitated with trichloroacetic acid (TCA) and subjected to Western blot analysis (anti-His antibody, Clontech). anti-Hissuggested: NoneExperimental Models: Cell Lines Sentences Resources Preparation of viral 5’-UTR mRNA and reporter RLuc mRNA: Preparation of HeLa translation-competent lysates: HeLa S3 lysates were prepared similarly as described before15. HeLasuggested: NoneFor this, frozen HEK293E cells were thawed and resuspended in 2x excess of lysis buffer (25 mM HEPES-KOH pH 7.6, 5 mM MgCl2, 50 mM KCl, cOmplete protease inhibitor cocktail (Roche), 1 mM PMSF, 0.2 U/µl RiboLock). HEK293Esuggested: NoneMicrographs (10’104 for the in vitro binding experiment, 16’887 for the HEK cell extract) were carefully inspected based on CTF estimations for drift and ice quality. HEKsuggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)Software and Algorithms Sentences Resources The plate was incubated at 30 °C for 10 min and the luminescence signal was measured three times using the TECAN infinite M100 Pro plate reader and plotted on GraphPad after performing three independent biological replicates. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)The super-resolution micrographs collected with the K3 camera were additionally binned 2 times during the MotionCor2 procedure. MotionCor2suggested: (MotionCor2, RRID:SCR_016499)Zenodo https://doi.org/10.5281/zenodo.3576630 (2019)). Zenodosuggested: (ZENODO, RRID:SCR_004129)The coordinates were subjected to 5 cycles of real space refinement using PHENIX 1.1827. PHENIXsuggested: (Phenix, RRID:SCR_014224)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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