An infectious SARS-CoV-2 B.1.1.529 Omicron virus escapes neutralization by therapeutic monoclonal antibodies

  1. Laura A. VanBlargan
  2. John M. Errico
  3. Peter J. Halfmann
  4. Seth J. Zost
  5. James E. Crowe
  6. Lisa A. Purcell
  7. Yoshihiro Kawaoka
  8. Davide Corti
  9. Daved H. Fremont
  10. Michael S. Diamond

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Abstract

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  1. Version published to 10.1038/s41591-021-01678-y
  2. Version published to 10.21203/rs.3.rs-1175516/v1 on Research Square
  3. ScreenIT

    SciScore for 10.1101/2021.12.15.472828: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line AuthenticationContamination: All cells routinely tested negative for mycoplasma using a PCR-based assay.

    Table 2: Resources

    Antibodies
    SentencesResources
    Monoclonal antibody purification: The mAbs used in this paper (COV2-2196, COV2-2130, S309, REGN10933, REGN10987, LY-CoV555, LY-CoV016, CT-P59, SARS2-38) have been described previously12,15,19,42-46.
    COV2-2130
    suggested: None
    SARS2-38
    suggested: None
    Antibody-virus complexes were added to Vero-TMPRSS2 or Vero-hACE2-TMPRSS2 cell monolayers in 96-well plates and incubated at 37°C for 1 h.
    Vero-TMPRSS2
    suggested: None
    Plates with WA1/2020 D614G were washed and sequentially incubated with an oligoclonal pool of SARS2-2, SARS2-11, SARS2-16, SARS2-31, SARS2-38, SARS2-57, and SARS2-7147 anti-S antibodies.
    SARS2-57
    suggested: None
    SARS2-7147
    suggested: None
    anti-S
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Cells: Vero-TMPRSS239 and Vero-hACE2-TMPRRS26 cells were cultured at 37°C in Dulbecco’s Modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 10LmM HEPES pH 7.3, and 100LU/ml of penicillin–streptomycin.
    Vero-hACE2-TMPRRS26
    suggested: None
    Vero-hACE2-TMPRSS2 cells were supplemented with 10 µg/mL of puromycin.
    Vero-hACE2-TMPRSS2
    suggested: None
    The B.1.1.529 isolate (hCoV-19/USA/WI-WSLH-221686/2021) was obtained from a midturbinate nasal swab and passaged once on Vero-TMPRSS2 cells as described41.
    Vero-TMPRSS2
    suggested: JCRB Cat# JCRB1818, RRID:CVCL_YQ48)
    Software and Algorithms
    SentencesResources
    Antibody-dose response curves were analyzed using non-linear regression analysis with a variable slope (GraphPad Software), and the half-maximal inhibitory concentration (EC50) was calculated.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 19. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2021.12.15.472828 on bioRxiv