Immune responses to two and three doses of the BNT162b2 mRNA vaccine in adults with solid tumors
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SciScore for 10.1101/2021.05.13.21257129: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Participant Selection: This protocol was approved by the University of Arizona Institutional Review Board and activated in January 2021.
Consent: Participants scheduled for vaccine appointments at Banner University Medical Center North site were approached with the IRB approved consent and sequentially enrolled.Sex as a biological variable Cell pellets were resuspended in 1 mL of X-VIVO 15 media with Gentamicin and Phenol Red (VWR #12001-988) containing 5% male human AB serum (Sigma #H4522-100ML), and incubated in 24-well plates overnight at 37°C with 5% CO2. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
… SciScore for 10.1101/2021.05.13.21257129: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Participant Selection: This protocol was approved by the University of Arizona Institutional Review Board and activated in January 2021.
Consent: Participants scheduled for vaccine appointments at Banner University Medical Center North site were approached with the IRB approved consent and sequentially enrolled.Sex as a biological variable Cell pellets were resuspended in 1 mL of X-VIVO 15 media with Gentamicin and Phenol Red (VWR #12001-988) containing 5% male human AB serum (Sigma #H4522-100ML), and incubated in 24-well plates overnight at 37°C with 5% CO2. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Plates were then washed and incubated for 1hr in 1% PBS and milk containing anti-human Pan-Ig HRP conjugated antibody (Jackson ImmunoResearch catalog 109-035-064) at a concentration of 1:2000 for 1 h. anti-human Pan-Ig HRPsuggested: NonePlates were emptied, washed 5 times with 200 μl/well of PBS (Fisher Scientific Hyclone PBS #SH2035), and incubated for 2 hours at room temperature with 100 μl/well PBS containing 0.5% fetal calf serum and 1 μg/ml detection antibody (7-B6-1-biotin). 7-B6-1-biotinsuggested: NoneExperimental Models: Cell Lines Sentences Resources Virus neutralization assays: SARS Coronavirus 2, Isolate USA-WA1/2020 (BEI NR-52281) was passaged once on Vero (ATCC #CCL-81) cells at a multiplicity of infection of 0.01 for 48 hours. Verosuggested: ATCC Cat# CCL-81, RRID:CVCL_0059)S1 and RBD tetramers were validated by staining 293T cells as a negative control or 293T-hACE2-expressing cells72 (BEI Resources NR-52511) as a positive control. 293Tsuggested: NoneSoftware and Algorithms Sentences Resources Tetramer reagents were assembled by mixing 100μg/ml C-terminal Avitagged RBD or S1 (AcroBiosystems) with 100μg/ml streptavidin-PE (eBiosciences) or streptavidin-BV421 (Biolegend), respectively, at a 5:1 molar ratio in which 1/10 the final volume of streptavidin was added every 5 minutes. AcroBiosystemssuggested: (ACRObiosystems, RRID:SCR_012550)Data were analyzed using FlowJo software. FlowJosuggested: (FlowJo, RRID:SCR_008520)All analyses were performed in GraphPad Prism 9 and the R programming language version 4.0.5. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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