A human three-dimensional neural-perivascular ‘assembloid’ promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology

  1. Lu Wang
  2. David Sievert
  3. Alex E. Clark
  4. Sangmoon Lee
  5. Hannah Federman
  6. Benjamin D. Gastfriend
  7. Eric V. Shusta
  8. Sean P. Palecek
  9. Aaron F. Carlin
  10. Joseph G. Gleeson

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Abstract

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  1. Version published to 10.1038/s41591-021-01443-1
  2. ScreenIT

    SciScore for 10.1101/2021.02.09.430349: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIACUC: SARS-CoV-2 infection of pericyte-like cells, COs, and PCCOs and Plaque assay: All work with SARS-CoV-2 was conducted in Biosafety Level-3 conditions at the University of California San Diego following the guidelines approved by the Institutional Biosafety Committee.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variableHuman Induced Pluripotent Stem Cells, Neural Crest Stem Cells Culture, and constructs: HEK293T cells (sex typed as female), Hela and H1 ESC (sex typed as male) were obtained from ATCC (CRL-11268™), ATCC (ATCC®CCL-2TM) and WiCell (WAe001-A), and were not further authenticated.
    Cell Line AuthenticationAuthentication: Human Induced Pluripotent Stem Cells, Neural Crest Stem Cells Culture, and constructs: HEK293T cells (sex typed as female), Hela and H1 ESC (sex typed as male) were obtained from ATCC (CRL-11268™), ATCC (ATCC®CCL-2TM) and WiCell (WAe001-A), and were not further authenticated.
    Contamination: All cells were regularly mycoplasma negative.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Human Induced Pluripotent Stem Cells, Neural Crest Stem Cells Culture, and constructs: HEK293T cells (sex typed as female), Hela and H1 ESC (sex typed as male) were obtained from ATCC (CRL-11268™), ATCC (ATCC®CCL-2TM) and WiCell (WAe001-A), and were not further authenticated.
    HEK293T
    suggested: ATCC Cat# CRL-11268, RRID:CVCL_1926)
    SARS-CoV-2 isolate USA-WA1/2020 (BEI Resources) was propagated and infectious units quantified by plaque assay using Vero E6 (ATCC) cells.
    Vero E6
    suggested: RRID:CVCL_XD71)
    Software and Algorithms
    SentencesResources
    The images were further developed with IMARIS (Oxford Instruments).
    IMARIS
    suggested: (Imaris, RRID:SCR_007370)
    Proteins with significance over 15 were used for GO-Term and string analysis in goprofile (https://biit.cs.ut.ee/gprofiler/gost)and String (https://string-db.org/)
    String
    suggested: (STRING, RRID:SCR_005223)
    Quantification and statistical analysis: Statistical analysis was performed with the GraphPad Prism 8 software.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 13. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.21203/rs.3.rs-214352/v1 on Research Square
  4. Version published to 10.1101/2021.02.09.430349v1 on bioRxiv