Breadth of concomitant immune responses prior to patient recovery: a case report of non-severe COVID-19
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SciScore for 10.1101/2020.02.20.20025841: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Participants provided written informed consent prior to the study.
IRB: (HREC Reference number: HREC/17/MH/53 and HREC/15/MonH/64/2016.196) and University of Melbourne (ID #1442952.1 and #1443389.4) Human Research Ethics Committees.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Healthy donors D6-D10 were of a mean age of 32 (range 22-55 years; 40% females). Table 2: Resources
Antibodies Sentences Resources Whole blood staining and flow cytometry: Fresh whole blood (200μl per stain) was used to measure CD4+CXCR5+ICOS+PD1+ follicular T cells (Tfh) and CD3-CD19+CD27hiCD38hi antibody-secreting B cell (ASC; … SciScore for 10.1101/2020.02.20.20025841: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Participants provided written informed consent prior to the study.
IRB: (HREC Reference number: HREC/17/MH/53 and HREC/15/MonH/64/2016.196) and University of Melbourne (ID #1442952.1 and #1443389.4) Human Research Ethics Committees.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Healthy donors D6-D10 were of a mean age of 32 (range 22-55 years; 40% females). Table 2: Resources
Antibodies Sentences Resources Whole blood staining and flow cytometry: Fresh whole blood (200μl per stain) was used to measure CD4+CXCR5+ICOS+PD1+ follicular T cells (Tfh) and CD3-CD19+CD27hiCD38hi antibody-secreting B cell (ASC; plasmablast) populations as described3 as well as activated HLA-DR+CD38+CD8+ and HLA-DR+CD38+CD4+ T cells, inflammatory CD14+CD16+ and conventional CD14+ monocytes, activated HLA-DR+CD3-CD56+ NK cells, as per the specific antibody panels (Supplementary Table 1; gating strategy is presented in Supplementary Fig.1). CD3-CD19+CD27hiCD38hi antibody-secreting Bsuggested: Noneantibody-secretingsuggested: (Félix A. Rey; Pasteur Institute Cat# C10, RRID:AB_2725800)Detection of IgG and IgM antibodies in SARS-CoV-2 -infected vero cells: Immunofluorescence antibody tests for the detection of IgG and IgM were performed using SARS-CoV-2-infected vero cells that had been washed with PBS and methanol/acetone fixed onto glass slides. IgMsuggested: NonePrior to detection of IgM antibodies, samples were pre-treated with RF-SorboTech (Alere, Rűsselsheim, Germany) to remove IgG antibodies and rheumatoid factors, which may cause false-negative and false-positive IgM results, respectively. remove IgGsuggested: NoneSoftware and Algorithms Sentences Resources Flow cytometry data were analyzed using FlowJo v10 software. FlowJosuggested: (FlowJo, RRID:SCR_008520)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 10. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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