Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
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SciScore for 10.1101/2021.03.08.434228: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Ethics: The study protocol for acquisition of human tissue samples conformed to the ethical guidelines of the 1975 Declaration of Helsinki and was reviewed by the ethics review board at the University of Alberta (Pro00005105, Pro00085859 and Pro00035875). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Lectin, Cholera toxin, and anti-ACE2 antibody staining by flow cytometry: Approximately 50,000 cells were seeded in a 96-well U-bottom flask and spun at 300 rcf for 5 min. Lectin , Cholera toxinsuggested: Noneanti-…SciScore for 10.1101/2021.03.08.434228: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Ethics: The study protocol for acquisition of human tissue samples conformed to the ethical guidelines of the 1975 Declaration of Helsinki and was reviewed by the ethics review board at the University of Alberta (Pro00005105, Pro00085859 and Pro00035875). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Lectin, Cholera toxin, and anti-ACE2 antibody staining by flow cytometry: Approximately 50,000 cells were seeded in a 96-well U-bottom flask and spun at 300 rcf for 5 min. Lectin , Cholera toxinsuggested: Noneanti-ACE2 antibody staining by flow cytometry: Approximately 50,000 cells were seeded in a 96-well U-bottom flask and spun at 300 rcf for 5 minsuggested: Noneanti-ACE2suggested: NoneFor ACE2 staining, cells were resuspended in Goat anti-Human ACE2 antibody (1:500, R&D Systems) in the flow buffer. anti-Human ACE2suggested: (LSBio (LifeSpan Cat# LS-C347-500, RRID:AB_1271966)The pellets were resuspended in a solution of anti-goat AF647 secondary antibody (Thermo Fischer) and incubated on ice for another 30 min. anti-goat AF647suggested: (SouthernBiotech Cat# 6425-31, RRID:AB_2796349)Experimental Models: Cell Lines Sentences Resources Control Lentivirus Transduction: HEK293T cells expressing ACE2 (150,000)45 were plated in triplicate in a 24-well plate in 250 μL of DMEM growth media (Gibco), containing 10% HEK293Tsuggested: NoneImmunofluorescent cell staining: Vero-E6 cells grown on coverslips were analyzed by immunofluorescent staining. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources Data was processed using FlowJo® Software Version 9.9.6 FlowJo®suggested: (FlowJo, RRID:SCR_008520)LAS Application Suite X was used as well as ImageJ for the addition of the scale bars. ImageJsuggested: (ImageJ, RRID:SCR_003070)Where indicated, cells were treated with 2 mU of Vibrio cholerae (Sigma; #11080725001) or Arthrobacter ureafaciens (Sigma; #10269611001 or NEB; P0722) neuraminidase in 10 mM potassium acetate and 2.5 mg mL−1 Triton X-100, pH 4.2 at 37 °C O/N. Sigmasuggested: (SigmaPlot, RRID:SCR_003210)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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