Accelerated RNA detection using tandem CRISPR nucleases

Article activity feed

1. Version published to 10.1038/s41589-021-00842-2

   Aug 5, 2021
2. 

   ScreenIT

   Mar 26, 2021

   SciScore for 10.1101/2021.03.19.21253328: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   This kinetic scheme was then converted into a system of ordinary differential equations modeling the rate of change in the concentration of each reaction component as a function of time and the concentrations of the components using Mathematica.	Mathematica suggested: (Wolfram Mathematica, RRID:SCR_014448)
   Mass spectrometry data acquisition and processing were performed using MassLynx software (version 4.1, Waters).	MassLynx suggested: (MassLynx , RRID:SCR_014271)
   Limit-of-detection …

   SciScore for 10.1101/2021.03.19.21253328: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   This kinetic scheme was then converted into a system of ordinary differential equations modeling the rate of change in the concentration of each reaction component as a function of time and the concentrations of the components using Mathematica.	Mathematica suggested: (Wolfram Mathematica, RRID:SCR_014448)
   Mass spectrometry data acquisition and processing were performed using MassLynx software (version 4.1, Waters).	MassLynx suggested: (MassLynx , RRID:SCR_014271)
   Limit-of-detection analysis for LbuCas13-TtCsm6: For determination of limit of detection using the triplicate data in Fig. 3C, a one-tailed t-test with Welch’s correction was used to compare mean fluorescence fold-change of a positive reaction (with initial point being t = 6 min) to that of the control without RNA target, using GraphPad Prism 9.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)
   Custom control of the imaging hardware was implemented in MATLAB (2020a), using Thorlabs drivers and SDK (ThorCam) to control the camera acquisition, and serial communication to an Arduino Bluefruit Feather board to electronically trigger the LED illumination.	MATLAB suggested: (MATLAB, RRID:SCR_001622)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2021.03.19.21253328 on medRxiv

   Mar 24, 2021