Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection
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SciScore for 10.1101/2020.08.17.20176925: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study Approval of Patient Samples: All patient samples were obtained using protocols approved by the UCSF, Stanford University, and Rockefeller University Institutional Review Boards and in accordance with the Declaration of Helsinki. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources After 3 washes, secondary anti-Fab, anti-IgG, or anti-IgM antibody was added and incubated for 30 minutes before the addition of TMB for 3 minutes. anti-Fab, anti-IgG,suggested: Noneanti-IgMsuggested: NoneLittle difference in detection sensitivity was observed, except … SciScore for 10.1101/2020.08.17.20176925: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study Approval of Patient Samples: All patient samples were obtained using protocols approved by the UCSF, Stanford University, and Rockefeller University Institutional Review Boards and in accordance with the Declaration of Helsinki. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources After 3 washes, secondary anti-Fab, anti-IgG, or anti-IgM antibody was added and incubated for 30 minutes before the addition of TMB for 3 minutes. anti-Fab, anti-IgG,suggested: Noneanti-IgMsuggested: NoneLittle difference in detection sensitivity was observed, except that the (L5 + S15) and (L5 + S25) linker combinations resulted in slightly decreased sensitivity at low antibody concentrations. S15suggested: NoneBoth the recombinant anti-S antibody C004 and the commercial anti-N antibody (Sino biological, Cat#40588-T62–50) produced linear dose-dependent signal in this buffer (Figure 1B and C), which can be used to generate standard curves and calibrate the instruments for the spLUC assay. anti-Ssuggested: Noneanti-Nsuggested: NoneSoftware and Algorithms Sentences Resources Data and Statistical analysis: All graphing and statistical analysis was performed in GraphPad Prism. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)The non-parametric Spearman correlation analysis was used in Prism to determine the correlation R value between datasets. Prismsuggested: (PRISM, RRID:SCR_005375)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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