Biological activity-based modeling identifies antiviral leads against SARS-CoV-2
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SciScore for 10.1101/2020.07.27.223578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization For each model, compounds were randomly split into two groups of approximately equal sizes, one used for training and the other for testing. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After an incubation at 37⍰°C for 24⍰h, 2.5 μL/well of detection reagent mixture of two labeled anti-ZIKV NS1 antibodies was added to assay plates. anti-ZIKV NS1suggested: NoneExperimental Models: Cell Lines Sentences Resources SARS-CoV-2 cytopathic effect (CPE) assay: Vero-E6 cells previously selected for high ACE2 expression 27 … SciScore for 10.1101/2020.07.27.223578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization For each model, compounds were randomly split into two groups of approximately equal sizes, one used for training and the other for testing. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After an incubation at 37⍰°C for 24⍰h, 2.5 μL/well of detection reagent mixture of two labeled anti-ZIKV NS1 antibodies was added to assay plates. anti-ZIKV NS1suggested: NoneExperimental Models: Cell Lines Sentences Resources SARS-CoV-2 cytopathic effect (CPE) assay: Vero-E6 cells previously selected for high ACE2 expression 27 (grown in EMEM, 10% FBS, and 1% Penicillin/Streptomycin) were cultured in T175 flasks and passaged at 95% confluency. Vero-E6suggested: NoneThe plates were transported into the BSL-3 facility were a 25 μL aliquot of virus inoculated cells (4000 Vero E6 cells/well) was added to each well in columns 3-24. Vero E6suggested: NoneNS1 TR-FRET assay: HEK293 cells were maintained in EMEM medium with 10% fetal bovine serum, 1% pen/strep (Gibco, Cat. # 15140–122). HEK293suggested: NoneSoftware and Algorithms Sentences Resources For activity-based models, only compounds that showed activity in at least 10% of the Sytravon, Genesis or MLS assay panel were kept for analyses. Genesissuggested: (Genesis, RRID:SCR_015775)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.07.27.223578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Antibodies Sentences Resources After an incubation at 37M°C for 24Mh, 2.5 µL/well of detection reagent mixture of two labeled anti-ZIKV NS1 antibodies was added to assay plates. anti-ZIKV NS1suggested: NoneExperimental Models: Cell Lines Sentences Resources The plates were transported into the BSL-3 facility were a 25 μL aliquot of virus inoculated cells (4000 Vero E6 cells/well) was added to each well in columns 3-24. Vero E6suggested: None…SciScore for 10.1101/2020.07.27.223578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Antibodies Sentences Resources After an incubation at 37M°C for 24Mh, 2.5 µL/well of detection reagent mixture of two labeled anti-ZIKV NS1 antibodies was added to assay plates. anti-ZIKV NS1suggested: NoneExperimental Models: Cell Lines Sentences Resources The plates were transported into the BSL-3 facility were a 25 μL aliquot of virus inoculated cells (4000 Vero E6 cells/well) was added to each well in columns 3-24. Vero E6suggested: None2 NS1 TR-FRET assay HEK293 cells were maintained in EMEM medium with 10% fetal bovine serum, 1% pen/strep (Gibco, Cat. # 15140–122). HEK293suggested: NoneSoftware and Algorithms Sentences Resources A NCATS in- house collection, the Genesis library, of ~90K diverse compounds was also screened for NS1 activity at a single concentration (14 µM). Genesissuggested: (Genesis, RRID:SCR_015775)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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SciScore for 10.1101/2020.07.27.223578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Antibodies Sentences Resources After an incubation at 37M°C for 24Mh, 2.5 µL/well of detection reagent mixture of two labeled anti-ZIKV NS1 antibodies was added to assay plates. anti-ZIKV NS1suggested: NoneExperimental Models: Cell Lines Sentences Resources Materials and Methods Vero-E6 cells previously selected for high ACE2 expression (grown in EMEM, 10% FBS, and 1% Penicillin/Streptomycin) were cultured in T175 flasks and passaged at 95% confluency. Vero-E6suggested: NoneThe plates were transported into … SciScore for 10.1101/2020.07.27.223578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Antibodies Sentences Resources After an incubation at 37M°C for 24Mh, 2.5 µL/well of detection reagent mixture of two labeled anti-ZIKV NS1 antibodies was added to assay plates. anti-ZIKV NS1suggested: NoneExperimental Models: Cell Lines Sentences Resources Materials and Methods Vero-E6 cells previously selected for high ACE2 expression (grown in EMEM, 10% FBS, and 1% Penicillin/Streptomycin) were cultured in T175 flasks and passaged at 95% confluency. Vero-E6suggested: NoneThe plates were transported into the BSL-3 facility were a 25 μL aliquot of virus inoculated cells (4000 Vero E6 cells/well) was added to each well in columns 3-24. Vero E6suggested: None2 NS1 TR-FRET assay HEK293 cells were maintained in EMEM medium with 10% fetal bovine serum, 1% pen/strep (Gibco, Cat. # 15140–122). HEK293suggested: NoneSoftware and Algorithms Sentences Resources Two NCATS in-house diverse compound libraries, Sytravon, which contains ~44,000 compounds, and Genesis, which contains ~90,000 compounds, and a subset (~100,000 compounds) of the other NCATS bioactive libraries and a large diverse compound library (MLS), were also screened in subpanels of the NCATS assay portfolio. Genesissuggested: (Genesis, SCR_015775)Data from additional tools added to each annotation on a weekly basis.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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