SARS-CoV-2 uses a multipronged strategy to impede host protein synthesis
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SciScore for 10.1101/2020.11.25.398578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The Institutional Biosafety Committee of Weizmann Institute approved the protocol used in these studies. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cells and viruses: Calu3 cells (ATCC HTB-55) were cultured in 6-well or 10cm plates with RPMI supplemented with 10% fetal bovine serum (FBS) Calu3suggested: ATCC Cat# HTB-55, RRID:CVCL_0609)It was propagated (5 passages) and tittered on Vero E6 cells and then sequenced 9 before it was used. Vero E6suggested: RRID:CVCL_XD71)293T cells were … SciScore for 10.1101/2020.11.25.398578: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The Institutional Biosafety Committee of Weizmann Institute approved the protocol used in these studies. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cells and viruses: Calu3 cells (ATCC HTB-55) were cultured in 6-well or 10cm plates with RPMI supplemented with 10% fetal bovine serum (FBS) Calu3suggested: ATCC Cat# HTB-55, RRID:CVCL_0609)It was propagated (5 passages) and tittered on Vero E6 cells and then sequenced 9 before it was used. Vero E6suggested: RRID:CVCL_XD71)293T cells were transfected using JetPEI (Polyplus-transfection) following the manufacturer’s instructions. 293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Software and Algorithms Sentences Resources Alignment was performed using Bowtie v1.1.2 52 with maximum two mismatches per read. Bowtiesuggested: (Bowtie, RRID:SCR_005476)Junctions spanning reads were quantified using STAR 2.5.3a aligner 53, with running flags as suggested by 44, to overcome filtering of non-canonical junctions. STARsuggested: (STAR, RRID:SCR_015899)Pathway enrichment analysis: Enrichment analysis of cellular pathways in specific gene clusters was done with PANTHER version 15.0, with default settings and the PANTHER pathways data set 54,55. PANTHERsuggested: (PANTHER, RRID:SCR_004869)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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