Sensitivity of SARS-CoV-2 B.1.1.7 to mRNA vaccine-elicited antibodies
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Abstract
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SciScore for 10.1101/2021.01.19.21249840: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the East of England – Cambridge Central Research Ethics Committee (17/EE/0025). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial …
SciScore for 10.1101/2021.01.19.21249840: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study was approved by the East of England – Cambridge Central Research Ethics Committee (17/EE/0025). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2021.01.19.21249840: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement The study was approved by the East of England – Cambridge Central Research Ethics Committee (17/EE/0025). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Pseudotype virus preparation Viral vectors were prepared by transfection of 293T cells by using Fugene HD transfection reagent (Promega) 293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Results from OddPub: We did not …
SciScore for 10.1101/2021.01.19.21249840: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement The study was approved by the East of England – Cambridge Central Research Ethics Committee (17/EE/0025). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Pseudotype virus preparation Viral vectors were prepared by transfection of 293T cells by using Fugene HD transfection reagent (Promega) 293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
Limitations Our study was limited by relatively small sample size. Although the Spike pseudotyping system has been shown to faithfully represent full length infectious virus, there may be determinants outside the Spike that influence antibody susceptibility either directly or indirectly in a live replication competent system. On the other hand live virus systems allow replication and therefore mutation to occur, and rigorous sequencing at multiple steps is needed. In addition, the cells used in such live virus systems are monkey cells engineered to overexpress receptors as in the case of our work. Finally, T cell responses are not accounted for in the present study. ID Age Mean ID50 Fold Fold (SD) WT Change Change Δ69/70 B.1.1.7 501Y Spike 570D mutations 1 60-65 34 (21) 0.435739 1.78 2 80-85 105 (62) 1.041735 2.18 3 40-45 93 (73) 0.32497 2.68 4 85-90 28 (15) 1.55961 - 5 25-30 27 (7) 0.386996 4.80 6 35-40 9 (0) 0.183133 4.46 7 80-85 27 (11) 0.383294 5.83 8 80-85 1 (0) - - 9 55-60 834 (658) 2.88 10 85-90 1 (0) 0.58253 - 11 80-85 2 (1) - - 12 80-85 3 (2) - - 13 80-85 4 (1) - - 14 85-90 41 (16) 15 75-80 233 (104) 0.468458 NA 4.07025411 6.12008534 16 80-85 1 (0) - - 17 80-85 1 (0) - - 18 85-90 723 (385) 0.674522 3.62182971 19 85-90 29 (7) 0.230936 5.41319667 20 80-85 63 (44) 0.538738 3.85473562 22 55-60 41 (17) 0.395114 5.27833669 25 60-65 52 (12) 0.808271 2.34132055 28 80-82 3449 (2489) 0.60931 3.17481581 - Table 1: Participants and serum neutralisation data; ID50 (inhibitory dil...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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