A mouse-adapted model of SARS-CoV-2 to test COVID-19 countermeasures
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SciScore for 10.1101/2020.05.06.081497: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Human tracheobronchial epithelial cells provided by Dr. Scott Randell were obtained from airway specimens resected from patients undergoing surgery under University of North Carolina Institutional Review Board-approved protocols (#03-1396) by the Cystic Fibrosis Center Tissue Culture Core.
IACUC: All animal work was approved by Institutional Animal Care and Use Committee at University of North Carolina at Chapel Hill under protocol 19-168 according to guidelines outlined by the Association for the Assessment and Accreditation of Laboratory Animal Care and the U.S. Department of Agriculture.Randomization not detected. Blinding not detected. Power Analysis not … SciScore for 10.1101/2020.05.06.081497: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Human tracheobronchial epithelial cells provided by Dr. Scott Randell were obtained from airway specimens resected from patients undergoing surgery under University of North Carolina Institutional Review Board-approved protocols (#03-1396) by the Cystic Fibrosis Center Tissue Culture Core.
IACUC: All animal work was approved by Institutional Animal Care and Use Committee at University of North Carolina at Chapel Hill under protocol 19-168 according to guidelines outlined by the Association for the Assessment and Accreditation of Laboratory Animal Care and the U.S. Department of Agriculture.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Serial sections were stained with hematoxylin and eosin, and stained for immunohistochemistry for SARS-CoV-2 nucleocapsid using a monoclonal anti-SARS-CoV-1 nucleocapsid antibody (NB100-56576, Novus Biologicals) on deparaffinized sections on the Ventana Discovery Ultra platform (Roche). anti-SARS-CoV-1suggested: NoneExperimental Models: Cell Lines Sentences Resources Vero E6 cells were maintained in n Dulbecco’s modified Eagle’s medium (DMEM; Gibco), 5% Fetal Clone II serum (FCII, Hyclone), and 1X antibiotic/antimycotic (Gibco). Vero E6suggested: RRID:CVCL_XD71)Experimental Models: Organisms/Strains Sentences Resources BALB/c mice were obtained from Envigo (strain 047). BALB/csuggested: NoneSoftware and Algorithms Sentences Resources Structure modelling was performed using Modeller (Version 9.20) and visualized using PyMOL (Version 1.8.6.0). Modellersuggested: (MODELLER, RRID:SCR_008395)PyMOLsuggested: (PyMOL, RRID:SCR_000305)Pegylated-IFN-λ1 treatment in vitro and in vivo: Peginterferon Lambda-1a was obtained from Eiger BioPharmaceuticals by MTA in GMP prefilled syringes, 0.18 mg/syringe (0.4 mg/mL). Eiger BioPharmaceuticalssuggested: NonePhotomicrographs were captured on an Olympus BX43 light microscope at 200X magnification with a DP27 camera using cellSens Dimension software. cellSenssuggested: NoneData analysis and presentation: All data visualize and analyzed in Prism (version 8.4.2). Prismsuggested: (PRISM, RRID:SCR_005375)Figures arranged in Adobe Illustrator (version 24.1). Adobe Illustratorsuggested: (Adobe Illustrator, RRID:SCR_010279)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04331899 Active, not recruiting Single-Blind Study of a Single Dose of Peginterferon Lambda-… NCT04343976 Enrolling by invitation Pegylated Interferon Lambda Treatment for COVID-19 NCT04344600 Recruiting Peginterferon Lambda-1a for the Prevention and Treatment of … NCT04354259 Recruiting Interferon Lambda for Immediate Antiviral Therapy at Diagnos… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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