Respiratory disease in rhesus macaques inoculated with SARS-CoV-2
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SciScore for 10.1101/2020.03.21.001628: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Ethics and biosafety statement: All animal experiments were approved by the Institutional Animal Care and Use Committee of Rocky Mountain Laboratories, NIH and carried out by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International accredited facility, according to the institution’s guidelines for animal use, following the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act, United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals. Randomization not detected. B… SciScore for 10.1101/2020.03.21.001628: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Ethics and biosafety statement: All animal experiments were approved by the Institutional Animal Care and Use Committee of Rocky Mountain Laboratories, NIH and carried out by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International accredited facility, according to the institution’s guidelines for animal use, following the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act, United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals. Randomization not detected. Blinding Histopathological analysis of tissue slides was performed by a board-certified veterinary pathologist blinded to the group assignment of the animals. Power Analysis not detected. Sex as a biological variable Study design: To evaluate the use of rhesus macaques as a model for SARS-CoV-2, eight adult rhesus macaques (4 males, 4 females) were inoculated via a combination of intranasal (0.5ml per nostril), intratracheal ( Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources To detect SARS-CoV-2 antigen, immunohistochemistry was performed using an anti-SARS nucleocapsid protein antibody (Novus Biologicals) at a 1:250 dilution. anti-SARS nucleocapsid proteinsuggested: NoneSARS-CoV-2-specific antibodies were detected using anti-monkey IgG polyclonal antibody HRP-conjugated antibody (KPL), peroxidase-substrate reagent (KPL) and stop reagent (KPL) anti-monkey IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources VeroE6 cells were maintained in DMEM supplemented with 10% fetal calf serum, 1 mM L-glutamine, 50 U/ml penicillin and 50 μg/ml streptomycin. VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.03.21.001628: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Methods Ethics and biosafety statement All animal experiments were approved by the Institutional Animal Care and Use Committee of Rocky Mountain Laboratories , NIH and carried out by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care ( AAALAC ) International accredited facility , according to the institution’s guidelines for animal use , following the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals , the Animal Welfare Act , United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals . Randomiza… SciScore for 10.1101/2020.03.21.001628: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Methods Ethics and biosafety statement All animal experiments were approved by the Institutional Animal Care and Use Committee of Rocky Mountain Laboratories , NIH and carried out by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care ( AAALAC ) International accredited facility , according to the institution’s guidelines for animal use , following the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals , the Animal Welfare Act , United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals . Randomization not detected. Blinding Histopathological analysis of tissue slides was performed by a board-certified veterinary pathologist blinded to the group assignment of the animals . Power Analysis not detected. Sex as a biological variable Study design To evaluate the use of rhesus macaques as a model for SARS-CoV-2 , eight adult rhesus macaques ( 4 males , 4 females ) were inoculated via a combination of intranasal ( 0.5ml per 5 nostril) , intratracheal ( 4ml) Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources To detect SARS-CoV-2 antigen , immunohistochemistry was performed using an anti-SARS nucleocapsid protein antibody ( Novus Biologicals ) at a 1:250 dilution . anti-SARS nucleocapsid proteinsuggested: NoneSARS-CoV-2-specific antibodies were detected using anti-monkey IgG polyclonal antibody HRP-conjugated antibody ( KPL) , peroxidase-substrate reagent ( KPL ) and stop reagent ( KPL) anti-monkey IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources After 60 min incubation at 37 °C, virus:serum mixture was added to VeroE6 cells and incubated at 37°C and 5% CO2. VeroE6suggested: JCRB Cat# JCRB1819, CVCL_YQ49Software and Algorithms Sentences Resources Digital images were acquired with a Gatan Rio bottom mount digital camera system ( Gatan Inc . , Pleasanton , CA and processed using Adobe Photoshop v . Adobe Photoshopsuggested: (Adobe Photoshop, SCR_014199)This study was supported by the Intramural Research Program, NIAID, NIH. Author contributions VJM and EdW designed the study; VJM, FF,BW, NvD, LPP, JS, KMW, AO, JC, BB, VAA, RR, PH, GS, EF, DS and EdW acquired, analyzed and interpreted the data; VJM, PH, EF, DS and EdW wrote the manuscript. Intramural Research Programsuggested: (Intramural Research Program, SCR_012734)Results from OddPub: Thank you for sharing your data.
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SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.
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