Daily longitudinal sampling of SARS-CoV-2 infection reveals substantial heterogeneity in infectiousness

Article activity feed

1. Version published to 10.1038/s41564-022-01105-z

   Apr 28, 2022
2. 

   ScreenIT

   Jul 14, 2021

   SciScore for 10.1101/2021.07.12.21260208: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: This study was approved by the Western Institutional Review Board, and all participants provided informed consent. Consent: This study was approved by the Western Institutional Review Board, and all participants provided informed consent.
   Sex as a biological variable	not detected.
   Randomization	Sample collection: Each day, participants were remotely observed by trained study staff collecting: The order of nostrils (left vs. right) used for the two different swabs was randomized.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus culture from nasal swabs: Vero-TMPRSS2 cells were grown …

   SciScore for 10.1101/2021.07.12.21260208: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: This study was approved by the Western Institutional Review Board, and all participants provided informed consent. Consent: This study was approved by the Western Institutional Review Board, and all participants provided informed consent.
   Sex as a biological variable	not detected.
   Randomization	Sample collection: Each day, participants were remotely observed by trained study staff collecting: The order of nostrils (left vs. right) used for the two different swabs was randomized.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Virus culture from nasal swabs: Vero-TMPRSS2 cells were grown in complete medium (CM) consisting of DMEM with 10% fetal bovine serum (Gibco), 1 mM glutamine (Invitrogen), 1 mM sodium pyruvate (Invitrogen), 100 U/ml of penicillin (Invitrogen), and 100 μg/ml of streptomycin (Invitrogen) (	Vero-TMPRSS2 suggested: JCRB Cat# JCRB1818, RRID:CVCL_YQ48)
   Software and Algorithms
   Sentences	Resources
   Swift v2 primer sequences were trimmed prior to variant analysis from iVar version 1.3.1 (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1618-7) retaining all calls with a minimum allele frequency of 0.01 and higher.	iVar suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a protocol registration statement.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.07.12.21260208v1 on medRxiv

   Jul 12, 2021