SARS-CoV-2 variant prediction and antiviral drug design are enabled by RBD in vitro evolution
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SciScore for 10.1101/2021.01.06.425392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Pseudo-virus production and inhibition of infection by RBD: Pseudo-virus production: SARS-CoV-2-Spike pseudotyped Lentivirus was produced by cotransfection of Hek293T cells pCMV ΔR8.2, pGIPZ-GFP, (26) and pCMV3 SΔC19 at a ratio of 1:1:1. 24 hours before the transfection 1 x 106 cells were seeded into a 10 cm plate. Hek293Tsuggested: NoneThe activity was measured on either purified ACE2 (0.75 ng; Abcam, aB351852) or the following cell … SciScore for 10.1101/2021.01.06.425392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Pseudo-virus production and inhibition of infection by RBD: Pseudo-virus production: SARS-CoV-2-Spike pseudotyped Lentivirus was produced by cotransfection of Hek293T cells pCMV ΔR8.2, pGIPZ-GFP, (26) and pCMV3 SΔC19 at a ratio of 1:1:1. 24 hours before the transfection 1 x 106 cells were seeded into a 10 cm plate. Hek293Tsuggested: NoneThe activity was measured on either purified ACE2 (0.75 ng; Abcam, aB351852) or the following cell lines – HeLa transiently transfected with ACE2 (6000 cells per assay), HEK-293T stable transfected with ACE2 (GenScript M00770 HeLasuggested: NoneHEK-293Tsuggested: None0, 8000 cells per assay), Caco2 cells (40,000 cells per assay). Caco2suggested: CLS Cat# 300137/p1665_CaCo-2, RRID:CVCL_0025)Software and Algorithms Sentences Resources The standard non-cooperative Hill equation was fitted by nonlinear least-squares regression using Python 3.7. Pythonsuggested: (IPython, RRID:SCR_001658)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 37 and 31. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2021.01.06.425392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The HEK-293T cells stably expressing hACE2 were pre-incubated with serial dilutions of the two RBDs for 1 h and then the pseudovirus was added for 48 hrs. HEK-293Tsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when …
SciScore for 10.1101/2021.01.06.425392: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The HEK-293T cells stably expressing hACE2 were pre-incubated with serial dilutions of the two RBDs for 1 h and then the pseudovirus was added for 48 hrs. HEK-293Tsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap used on pages 28 and 34. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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