Longitudinal observation and decline of neutralizing antibody responses in the three months following SARS-CoV-2 infection in humans
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SciScore for 10.1101/2020.07.09.20148429: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources This protein was expressed in 500 mL HEK-293F cells (Invitrogen) at a density of 1.5 million cells/mL. HEK-293Fsuggested: RRID:CVCL_6642)Virus preparation: Pseudotyped HIV virus incorporating the SARS-Cov2 spike protein was produced in a 10 cm dish seeded the day prior with 3.5×106 HEK293T/17 cells in 10 ml of complete Dulbecco’s Modified Eagle’s Medium (DMEM-C) containing 10% (vol/vol) HEK293T/17suggested: NoneNext, Hela cells stably … SciScore for 10.1101/2020.07.09.20148429: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources This protein was expressed in 500 mL HEK-293F cells (Invitrogen) at a density of 1.5 million cells/mL. HEK-293Fsuggested: RRID:CVCL_6642)Virus preparation: Pseudotyped HIV virus incorporating the SARS-Cov2 spike protein was produced in a 10 cm dish seeded the day prior with 3.5×106 HEK293T/17 cells in 10 ml of complete Dulbecco’s Modified Eagle’s Medium (DMEM-C) containing 10% (vol/vol) HEK293T/17suggested: NoneNext, Hela cells stably expressing the ACE2 receptor (provided by Dr James Voss, The Scripps Research Institute) were added and the plates were left for 72 hours. Helasuggested: NoneSoftware and Algorithms Sentences Resources Half-maximal binding (EC50) was calculated using GraphPad Prism. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.07.09.20148429: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The cohort were 77.2% male with average age of 55.2 years (range 23-95 years). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibody binding responses to SARS-CoV-2: The IgG, IgM and IgA response against spike (S), the receptor binding domain (RBD) and nucleocapsid (N) were measured by ELISA over multiple time points (Figure 1 and S1).6 Initially, the optical density at 1:50 serum dilution was measured for 300 samples from the 65 individuals (Figure 1 and S1). S1…SciScore for 10.1101/2020.07.09.20148429: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The cohort were 77.2% male with average age of 55.2 years (range 23-95 years). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibody binding responses to SARS-CoV-2: The IgG, IgM and IgA response against spike (S), the receptor binding domain (RBD) and nucleocapsid (N) were measured by ELISA over multiple time points (Figure 1 and S1).6 Initially, the optical density at 1:50 serum dilution was measured for 300 samples from the 65 individuals (Figure 1 and S1). S1suggested: NoneNeutralizing antibody responses to SARS-CoV-2: We next measured SARS-CoV-2 neutralization potency using HIV-1 (human immunodeficiency virus-1) based virus particles, pseudotyped with SARS-CoV-2 S17,18 in a HeLa cell line stably expressing the ACE2 receptor. HIV-1suggested: NoneExperimental Models: Cell Lines Sentences Resources Neutralizing antibody responses to SARS-CoV-2: We next measured SARS-CoV-2 neutralization potency using HIV-1 (human immunodeficiency virus-1) based virus particles, pseudotyped with SARS-CoV-2 S17,18 in a HeLa cell line stably expressing the ACE2 receptor. HeLasuggested: CLS Cat# 300194/p772_HeLa, CVCL_0030This protein was expressed in 500 mL HEK-293F cells (Invitrogen) at a density of 1.5 million cells/mL. HEK-293Fsuggested: CVCL_6642Virus preparation Pseudotyped HIV virus incorporating the SARS-Cov2 spike protein was produced in a 10 cm dish seeded the day prior with 3.5x106 HEK293T/17 cells in 10 ml of complete Dulbecco’s Modified Eagle’s Medium (DMEM-C) containing 10% (vol/vol) HEK293T/17suggested: ATCC Cat# CRL-11268, CVCL_1926Thank you to Florian Krammer for provision of the RBD expression plasmid, Philip Brouwer, Marit Van Gils and Rogier Sanders (University of Amsterdam) for the S protein construct, Leo James, Jakub Luptak and Leo Kiss (LMB) for the provision of purified N protein, and James Voss for providing the Hela-ACE2 cells. Hela-ACE2suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis Analyses were performed using R (version 4.0.0) and GraphPad Prism (version 7.0.4). GraphPad Prismsuggested: (GraphPad Prism, SCR_002798)Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
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