Detection of air and surface contamination by SARS-CoV-2 in hospital rooms of infected patients
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Abstract
Understanding the particle size distribution in the air and patterns of environmental contamination of SARS-CoV-2 is essential for infection prevention policies. Here we screen surface and air samples from hospital rooms of COVID-19 patients for SARS-CoV-2 RNA. Environmental sampling is conducted in three airborne infection isolation rooms (AIIRs) in the ICU and 27 AIIRs in the general ward. 245 surface samples are collected. 56.7% of rooms have at least one environmental surface contaminated. High touch surface contamination is shown in ten (66.7%) out of 15 patients in the first week of illness, and three (20%) beyond the first week of illness ( p = 0.01, χ 2 test). Air sampling is performed in three of the 27 AIIRs in the general ward, and detects SARS-CoV-2 PCR-positive particles of sizes >4 µm and 1–4 µm in two rooms, despite these rooms having 12 air changes per hour. This warrants further study of the airborne transmission potential of SARS-CoV-2.
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SciScore for 10.1101/2020.03.29.20046557: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Air sampling: Six NIOSH BC 251 bioaerosol samplers were placed in each of three AIIRs in the general ward to collect air samples. NIOSH BC 251suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Statistical analysis was performed using Stata version 15.1 (StataCorp, College Station, Texas) and GraphPad Prism 8.0 (GraphPad Software, Inc., San Diego). StataCorpsuggested: (Stata, RRID:SCR_012763)GraphPadsuggested:…SciScore for 10.1101/2020.03.29.20046557: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Air sampling: Six NIOSH BC 251 bioaerosol samplers were placed in each of three AIIRs in the general ward to collect air samples. NIOSH BC 251suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Statistical analysis was performed using Stata version 15.1 (StataCorp, College Station, Texas) and GraphPad Prism 8.0 (GraphPad Software, Inc., San Diego). StataCorpsuggested: (Stata, RRID:SCR_012763)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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