Coinfection with influenza A virus enhances SARS-CoV-2 infectivity
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SciScore for 10.1101/2020.10.14.335893: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mice were male, age-matched, and grouped for SARS-CoV-2 infection or IAV and SARS-CoV-2 co-infection. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Rabbit monoclonal antibody against ACE2 (Abclonal, A4612, 1:1000) A4612suggested: (ABclonal Cat# A4612, RRID:AB_2863309)mouse monoclonal antibody against SARS-CoV Nucleoprotein (Sino Biological, 40143-MM05, 1:1000), anti-actin (Abclonal, 1:1000), were purchased commercially. mouse monoclonal antibody against SARS-CoV Nucleoprotein ( Sino Biological , 40143-MM05 , 1:1000) …SciScore for 10.1101/2020.10.14.335893: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mice were male, age-matched, and grouped for SARS-CoV-2 infection or IAV and SARS-CoV-2 co-infection. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Rabbit monoclonal antibody against ACE2 (Abclonal, A4612, 1:1000) A4612suggested: (ABclonal Cat# A4612, RRID:AB_2863309)mouse monoclonal antibody against SARS-CoV Nucleoprotein (Sino Biological, 40143-MM05, 1:1000), anti-actin (Abclonal, 1:1000), were purchased commercially. mouse monoclonal antibody against SARS-CoV Nucleoprotein ( Sino Biological , 40143-MM05 , 1:1000) , anti-actin ( Abclonal , 1:1000)suggested: Noneantibody against SARS-CoV Nucleoprotein ( Sino Biological , 40143-MM05suggested: Noneanti-actinsuggested: NonePeroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly followed by image development with Chemiluminescent HRP Substrate Kit (Millipore Corporation). Antgene , 1: 5000suggested: NoneAntgene ,suggested: NoneThe primary antibodies used in this study were rabbit polyclonal antibody against ACE2 for immunofluorescence (Sino Biological, 10108-T26) and anti-influenza virus-NP (kindly provided by Prof. Ningshao Xia). ACE2suggested: Noneanti-influenza virus-NPsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cells and viruses: The 293T, A549, Huh-7, MDCK, and Vero E6, WI-38, WI-38 VA-13, and BEAS-2B were obtained from ATCC and maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% foetal bovine serum (FBS), Calu-3 (ATCC) was maintained in DMEM supplemented with 20% FBS. 293Tsuggested: NoneHuh-7suggested: NoneMDCKsuggested: NoneWI-38suggested: NoneBEAS-2Bsuggested: NoneCalu-3suggested: NoneNCI-H292(ATCC) was maintained with RPMI-1640 (Gibco) supplemented with 20% FBS. NCI-H292suggested: NoneForty-eight hours post-transfection, 150 μl pseudotyped VSV-ΔG bearing VSV-G protein were used to infect Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71). Immunofluorescence: A549 cells were fixed and incubated with primary antibodies. A549suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)Software and Algorithms Sentences Resources ACE2 knocking-down cells: Two sgRNAs targeting the hACE2 gene were designed under the protocol in http://chopchop.cbu.uib.no and commercially synthesized to clone in lenti-Cas9-blast vector (kindly provided by Prof. Hongbing Shu). http://chopchop.cbu.uib.nosuggested: (CHOPCHOP, RRID:SCR_015723)Mice: The K18 hACE2 transgenic mice purchased from Gempharmatech were housed in ABSL-3 pathogen-free facilities under 12-h light-dark cycles with access to food and water. Gempharmatechsuggested: (GemPharmatech, RRID:SCR_017239)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.10.14.335893: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mice 296 were male, age-matched, and grouped for SARS-CoV-2 infection or IAV and SARS-CoV- 297 2 co-infection. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 270 Rabbit monoclonal antibody against ACE2 (Abclonal, A4612, 1:1000) A4612suggested: (ABclonal Cat# A4612, RRID:AB_2863309)mouse monoclonal 271 antibody against SARS-CoV Nucleoprotein (Sino Biological, 40143-MM05, 1:1000) … SciScore for 10.1101/2020.10.14.335893: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mice 296 were male, age-matched, and grouped for SARS-CoV-2 infection or IAV and SARS-CoV- 297 2 co-infection. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources 270 Rabbit monoclonal antibody against ACE2 (Abclonal, A4612, 1:1000) A4612suggested: (ABclonal Cat# A4612, RRID:AB_2863309)mouse monoclonal 271 antibody against SARS-CoV Nucleoprotein (Sino Biological, 40143-MM05, 1:1000) antibody against SARS-CoV Nucleoprotein ( Sino Biological , 40143-MM05suggested: NoneThe primary antibodies used 278 in this study were rabbit polyclonal antibody against ACE2 for immunofluorescence (Sino 279 Biological, 10108-T26) and anti-influenza virus-NP (kindly provided by Prof. ACE2suggested: Noneanti-influenza virus-NPsuggested: None281 Alexa Fluor M555, Invitrogen) and DAPI (Beyotime, C1002), were admitted afterward Peroxidase-conjugated secondary antibodies according to standard protocols. C1002suggested: NoneExperimental Models: Cell Lines Sentences Resources 200 The 293T, A549, Huh-7, MDCK, and Vero E6, WI-38, WI-38 VA-13, and BEAS-2B were 201 obtained from ATCC and maintained in Dulbecco’s modified Eagle’s medium (DMEM; 202 Gibco) supplemented with 10% foetal bovine serum (FBS), Calu-3 (ATCC) was 203 maintained in DMEM supplemented with 20% FBS. 293Tsuggested: NoneHuh-7suggested: NoneWI-38suggested: NoneBEAS-2Bsuggested: NoneCalu-3suggested: BCRJ Cat# 0264, RRID:CVCL_0609)NCI-H292(ATCC) was maintained 204 with RPMI-1640 (Gibco) supplemented with 20% FBS. NCI-H292suggested: NoneTo produce 226 pseudotyped VSV-ΔG-Luc/mCherry bearing SARS-CoV-2 spike protein (pSARS-CoV-2), 227 Vero E6 cells were seeded in 10 cm dish and transfected simultaneously with 15 μg SARS- 228 CoV-2-S-Δ18 plasmid by Lipofectamine 3000 (Thermo). Vero E6suggested: NoneForty-eight hours post- 229 transfection, 150 µl pseudotyped VSV-ΔG bearing VSV-G protein were used to infect Vero 230 E6 cells. Vero 230suggested: None458 (J-L) WT A549, and IFNAR-/-A549 cells were infected with WSN at MOI 0.1 for 12 hours, 459 cells were then infected with pSARS-CoV-2 for another 24 hours followed by measuring 460 luciferase activity and mRNA expression levels of indicated genes. IFNAR-/-A549suggested: None464 A549 cells were infected with A/WSN/33 at indicated MOIs. A549suggested: NCI-DTP Cat# A549, RRID:CVCL_0023)( A-C) P values are from 480 unpaired One-way ANOVA. * 482 (A) MDCK cells were pre-infected with WSN (MOI=0.1), H1N1(MOI=1), or H3N2 483 (MOI=1) for 12 hours and were then infected with pSARS-CoV-2 for another 24 hours 484 followed by measuring luciferase activity. MDCKsuggested: NoneSoftware and Algorithms Sentences Resources 284 ACE2 knocking-down cells 285 Two sgRNAs targeting the hACE2 gene were designed under the protocol in 286 http://chopchop.cbu.uib.no and commercially synthesized to clone in lenti-Cas9-blast 287 vector (kindly provided by Prof. Hongbing Shu). http://chopchop.cbu.uib.nosuggested: (CHOPCHOP, RRID:SCR_015723)293 Mice 294 The K18 hACE2 transgenic mice purchased from Gempharmatech were housed in ABSL- 295 3 pathogen-free facilities under 12-h light-dark cycles with access to food and water. Gempharmatechsuggested: (GemPharmatech, RRID:SCR_017239)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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