Engineered trimeric ACE2 binds viral spike protein and locks it in “Three-up” conformation to potently inhibit SARS-CoV-2 infection
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SciScore for 10.1101/2020.08.31.274704: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HEK 293F cells (Invitrogen) were cultured in Freestyle medium (Gibco, Lot.2164683) at 37 °C under 6% CO2 in a CRYSTAL shaker (140 rpm). HEK 293Fsuggested: RRID:CVCL_6642)BLI): Cell lines, plasmids construction and virus: Human hepatoma Huh-7 cells were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China) Huh-7suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)Then, the protein-virus mixtures were added to … SciScore for 10.1101/2020.08.31.274704: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HEK 293F cells (Invitrogen) were cultured in Freestyle medium (Gibco, Lot.2164683) at 37 °C under 6% CO2 in a CRYSTAL shaker (140 rpm). HEK 293Fsuggested: RRID:CVCL_6642)BLI): Cell lines, plasmids construction and virus: Human hepatoma Huh-7 cells were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China) Huh-7suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336)Then, the protein-virus mixtures were added to Vero-E6 cells and incubated at 37°C for 1 hour. Vero-E6suggested: NoneSoftware and Algorithms Sentences Resources The genes of 3HB and foldon were synthesized by Genewiz, Suzhou, China. Genewizsuggested: (GENEWIZ, RRID:SCR_003177)The stacks were motion corrected with MotionCor2 59 and binned 2-fold, resulting in a pixel size of 1.087 Å/pixel. MotionCor2suggested: (MotionCor2, RRID:SCR_016499)After 2D classification with Relion, good particles were selected and subjected to two cycles of heterogeneous refinement without symmetry using cryoSPARC 66. cryoSPARCsuggested: (cryoSPARC, RRID:SCR_016501)The good particles were selected and subjected to Non-uniform Refinement (beta) with C1 symmetry, resulting in the 3D reconstruction for the whole structures that were further subjected to 3D classification, 3D auto-refinement and post-processing with Relion. Relionsuggested: (RELION, RRID:SCR_016274)The fitted atomic models were further manually adjusted with Coot 70. Cootsuggested: (Coot, RRID:SCR_014222)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 25. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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