The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro
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SciScore for 10.1101/2020.08.17.254979: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Mothers were informed consent.
IRB: This study was approved by the ethics committees of the Medical CenterRandomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibody against nucleocapsid protein of anti-SARS-CoV-2 N protein (Genscript, USA) and GAPDH of anti-GAPDH (Proteintech, USA) were used at 1:3000 dilutions. Antibody against nucleocapsid protein of anti-SARS-CoV-2 N proteinsuggested: Noneanti-GAPDHsuggested: NoneThe second antibody of HRP-conjugated affinipure Goat anti-mouse IgG (H+L) were diluted at … SciScore for 10.1101/2020.08.17.254979: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Mothers were informed consent.
IRB: This study was approved by the ethics committees of the Medical CenterRandomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibody against nucleocapsid protein of anti-SARS-CoV-2 N protein (Genscript, USA) and GAPDH of anti-GAPDH (Proteintech, USA) were used at 1:3000 dilutions. Antibody against nucleocapsid protein of anti-SARS-CoV-2 N proteinsuggested: Noneanti-GAPDHsuggested: NoneThe second antibody of HRP-conjugated affinipure Goat anti-mouse IgG (H+L) were diluted at 1:20000. anti-mouse IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cell lines, coronavirus, and key reagents: Vero E6 cells (American Type Culture Collection, Manassas, VA, USA) and A549 cells were grown in high-glucose-containing Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum(13). A549suggested: NoneBlocking assay: To study whether the inhibition effects of skimmed breastmilk on SARS-CoV-2 pseudovirus and GX_P2V by binding cell surface to block viral entry, Vero E6 cells were seeded at the 96 well plate with 20,000 cells/well. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Statistical analyses were analyzed using GraphPad Prism 8 software (GraphPad Software Inc. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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