A systemic and molecular study of subcellular localization of SARS-CoV-2 proteins
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SciScore for 10.1101/2020.08.02.233023: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibodies: Anti-Giantin (ab80864) for visualizing Golgi body, and anti-CoxIV (ab16056) for showing mitochondria were purchased from Abcam (Cambridge, MA). Anti-Giantinsuggested: (Abcam Cat# ab80864, RRID:AB_10670397)anti-CoxIVsuggested: (Abcam Cat# ab16056, RRID:AB_443304)Anti-Tubulin ( Anti-Tubulinsuggested: NoneAnti-FLAG antibody (monoclonal), M2, and the anti-SC35 antibody (S4045) were purchased from Sigma. Anti-FLAGsuggested: Noneanti-SC35suggested: NoneSciScore for 10.1101/2020.08.02.233023: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibodies: Anti-Giantin (ab80864) for visualizing Golgi body, and anti-CoxIV (ab16056) for showing mitochondria were purchased from Abcam (Cambridge, MA). Anti-Giantinsuggested: (Abcam Cat# ab80864, RRID:AB_10670397)anti-CoxIVsuggested: (Abcam Cat# ab16056, RRID:AB_443304)Anti-Tubulin ( Anti-Tubulinsuggested: NoneAnti-FLAG antibody (monoclonal), M2, and the anti-SC35 antibody (S4045) were purchased from Sigma. Anti-FLAGsuggested: Noneanti-SC35suggested: NoneS4045suggested: NoneImmunofluorescent assay (IFA): Immunostaining was performed on cells grown on coverslips after fixation with 1% paraformaldehyde (10 min at room temperature) and permeabilization in 0.2% Triton (20 min on ice) by sequential incubation with primary and Texas red (TR)-labeled secondary antibodies (Vector Laboratories, Burlingame, Calif.) for 30 min each (all solutions in PBS). Texas red (TR)-labeled secondary antibodiessuggested: NoneExperimental Models: Cell Lines Sentences Resources Plasmids and transfection: To show a protein’s location in the Hep-2 cells, the plasmid constructed from “molecular cloning” is co-transfected with one of the following plasmids that expresses a cell marker. Hep-2suggested: CLS Cat# 300397/p694_Hep-2, RRID:CVCL_1906)Software and Algorithms Sentences Resources http://www.addgene.org). http://www.addgene.orgsuggested: (Addgene, RRID:SCR_002037)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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