Single dose of BNT162b2 mRNA vaccine against SARS-CoV-2 induces high frequency of neutralising antibody and polyfunctional T-cell responses in patients with myeloproliferative neoplasms

  1. Patrick Harrington
  2. Hugues de Lavallade
  3. Katie J. Doores
  4. Amy O’Reilly
  5. Jeffrey Seow
  6. Carl Graham
  7. Thomas Lechmere
  8. Deepti Radia
  9. Richard Dillon
  10. Yogita Shanmugharaj
  11. Andreas Espehana
  12. Claire Woodley
  13. Jamie Saunders
  14. Natalia Curto-Garcia
  15. Jennifer O’Sullivan
  16. Kavita Raj
  17. Shahram Kordasti
  18. Michael H. Malim
  19. Claire N. Harrison
  20. Donal P. McLornan

Reviewed by

Read the full article

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1038/s41375-021-01300-7
  2. ScreenIT

    SciScore for 10.1101/2021.04.27.21256096: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    No key resources detected.

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.04.27.21256096v1 on medRxiv
  4. ScreenIT

    SciScore for 10.1101/2021.04.15.21255482: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: Study design and vaccine: From 17th December 2020 until 17th February 2021, patients with a known diagnosis of myeloid haematological malignancies presenting at Guy’s & St Thomas’ NHS Foundation Trust and eligible to receive a 30μg injection of SARS-COV-2 mRNA BNT162b2 vaccine were approached for informed consent.
    IRB: All patients gave informed consent, and the study protocol was approved by the regional research and ethics review board
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Directly conjugated monoclonal antibodies with the following specificities were used; CD3 BUV395 (clone SK37), CD4 PE (clone M-T477), CD45RO BV711, TNFα (clone MAB11)
    CD3
    suggested: (BD Biosciences Cat# 340571, RRID:AB_400474)
    CD45RO
    suggested: (BioLegend Cat# 304235, RRID:AB_11218600)
    TNFα
    suggested: None
    N-specific monoclonal antibody), CR3022 (0.2□µg□ml-1)
    CR3022
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Neutralization assay with SARS-CoV-2: HIV-1 (human immunodeficiency virus type-1) based virus particles, pseudotyped with SARS-CoV-2 Wuhan Spike were prepared in HEK-293T/17 cells and neutralization assays were conducted as previously described3.
    HEK-293T/17
    suggested: ATCC Cat# CRL-11268, RRID:CVCL_1926)
    Next, HeLa cells stably expressing the ACE2 receptor (provided by Dr James Voss, Scripps Research, La Jolla, CA) were added (12,500 cells/50µL per well) and the plates were left for 72 hours.
    HeLa
    suggested: None
    Software and Algorithms
    SentencesResources
    Intracellular cytokine flow cytometry assay: T cell functionality was assessed using intracellular cytokine staining after incubation with SARS-CoV-2 specific peptides covering the immunogenic domains of the Spike (S) protein (Miltenyi Biotech).
    SARS-CoV-2
    suggested: (BioLegend Cat# 944703, RRID:AB_2890874)
    T cell analysis was performed on a BD Fortessa cytometer and results processed using Flowjo version 10.5.
    Flowjo
    suggested: (FlowJo, RRID:SCR_008520)
    Statistical analysis was performed using Prism, version 8.
    Prism
    suggested: (PRISM, RRID:SCR_005375)
    EC50 values were calculated in GraphPad Prism.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  5. Version published to 10.1101/2021.04.15.21255482v1 on medRxiv