Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2021.02.28.433291: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc. (cat. 40592-VNAH) HEK 293suggested: NoneSoftware and Algorithms Sentences Resources Mass spectra were output from the DataAnalysis 5.3 (Bruker Daltonics) software and analyzed using MASH Explorer.64 Data Analysis: All data were processed and analyzed using Compass DataAnalysis 4.3/5.3 and MASH Explorer.64 Maximum Entropy algorithm (Bruker Daltonics) was used to deconvolute all mass spectra with the resolution set to 80,000 for the timsTOF Pro or … SciScore for 10.1101/2021.02.28.433291: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc. (cat. 40592-VNAH) HEK 293suggested: NoneSoftware and Algorithms Sentences Resources Mass spectra were output from the DataAnalysis 5.3 (Bruker Daltonics) software and analyzed using MASH Explorer.64 Data Analysis: All data were processed and analyzed using Compass DataAnalysis 4.3/5.3 and MASH Explorer.64 Maximum Entropy algorithm (Bruker Daltonics) was used to deconvolute all mass spectra with the resolution set to 80,000 for the timsTOF Pro or with instrument peak width set to 0.05 for the 12T FTICR. Compass DataAnalysissuggested: NoneFor peak picking, the sophisticated numerical annotation procedure (SNAP) algorithm from Bruker DataAnalysis 5.3 was used with a quality threshold of 0.5 and an S/N lower threshold of 3. SNAPsuggested: (SNAP, RRID:SCR_007936)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-