Validation and Invalidation of SARS-CoV-2 Papain-like Protease Inhibitors

Article activity feed

1. Version published to 10.1021/acsptsci.1c00240

   Jan 24, 2022
2. 

   ScreenIT

   Nov 19, 2021

   SciScore for 10.1101/2021.11.04.467342: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin antibiotics in a 37 °C incubator with 5% CO2. 96-well Greiner plate (catalog no. 655090) was seeded with 293T cells to overnight 70–90% confluency.	293T suggested: None
   Recombinant DNA
   Sentences	Resources
   SARS-CoV-2 PLpro expression and purification: SARS-CoV-2 papain-like protease (PLpro) gene (ORF 1ab 1564–1876) …

   SciScore for 10.1101/2021.11.04.467342: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	not detected.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin antibiotics in a 37 °C incubator with 5% CO2. 96-well Greiner plate (catalog no. 655090) was seeded with 293T cells to overnight 70–90% confluency.	293T suggested: None
   Recombinant DNA
   Sentences	Resources
   SARS-CoV-2 PLpro expression and purification: SARS-CoV-2 papain-like protease (PLpro) gene (ORF 1ab 1564–1876) from strain BetaCoV/Wuhan/WIV04/2019 with E. coli codon optimization was ordered from GenScript in the pET28b(+) vector.	pET28b(+ suggested: RRID:Addgene_67287)
   50 ng of pcDNA3-flipGFP-PLpro-T2A-mCherry plasmid and 50 ng of pcDNA3.1-SARS2-PLpro were used each well in the presence of transfection reagent TransIT-293 (Mirus) per manufacturer protocol.	pcDNA3-flipGFP-PLpro-T2A-mCherry suggested: None pcDNA3.1-SARS2-PLpro suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.11.04.467342v1 on bioRxiv

   Nov 5, 2021