Electrophysiological and Proarrhythmic Effects of Hydroxychloroquine Challenge in Guinea-Pig Hearts

  1. Gongxin Wang
  2. Chieh-Ju Lu
  3. Andrew W. Trafford
  4. Xiaohui Tian
  5. Hannali M Flores
  6. Piotr Maj
  7. Kevin Zhang
  8. Yanhong Niu
  9. Luxi Wang
  10. Yimei Du
  11. Xinying Ji
  12. Yanfang Xu
  13. Lin Wu
  14. Dan Li
  15. Neil Herring
  16. David Paterson
  17. Christopher L.-H. Huang
  18. Henggui Zhang
  19. Ming Lei
  20. Guoliang Hao

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Abstract

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  1. Version published to 10.1021/acsptsci.1c00166
  2. ScreenIT

    SciScore for 10.1101/2020.05.21.108605: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIACUC: Study Approval: All procedures were approved by The Institutional Animals Ethics Committees at Henan University, Kaifeng, China under and the national guidelines under which the institution operates, and also conform with the NIH Guide for the Care and Use of Laboratory Animals.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Single-cell electrophysiological recordings: Core human cardiac channels were heterologously expressed in recombinant HEK293 or CHO cell lines and studied using conventional patch clamp methods.
    CHO
    suggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)
    Perforated whole-cell recordings of KCNQ1/E1 and Cav1.2 were obtained following perforation with 200 μg/ml Amphotericin included in the pipette solution.
    Cav1.2
    suggested: None
    Software and Algorithms
    SentencesResources
    All currents were recorded using a MultiClamp 700A amplifier, 1550A digitizer and pCLAMP 10.6 software (Molecular devices, USA).
    pCLAMP
    suggested: (pClamp, RRID:SCR_011323)
    The HCQ structure was obtained from PubChem and prepared for docking using LigPrep.
    PubChem
    suggested: (PubChem, RRID:SCR_004284)
    LigPrep
    suggested: (Ligprep, RRID:SCR_016746)
    Poses from Glide, FlexX, and AutoDock Vina were also re-scored using RF-Score-VS.
    FlexX
    suggested: (FlexX, RRID:SCR_000186)
    AutoDock
    suggested: (AutoDock, RRID:SCR_012746)
    ECP signals were recorded at a 10 kHz sampling using synchronized 64 channel EMS64-USB-1003 recording systems and recorded using EMapRecord 5.0 software (MapingLab Ltd., UK) and stored in a computer for subsequent off-line analysis.
    EMapRecord
    suggested: None
    The conduction velocity (CV) was calculated from the difference in timing and the known distance between the recording points using EMapScope 5.0 software (MappingLab Ltd.).
    EMapScope
    suggested: None
    The cameras of the optical mapping system, LED lights and stimulator and ECG recording were simultaneously driven by an 8 channel TTL analog-digital converter and OMapRecord 4.0 software (MappingLab Ltd.).
    OMapRecord
    suggested: None
    For the analysis of optical mapping signals and generation of isochronal maps, data were semi-automatically processed using OMapScope 5.0 software (MappingLab Ltd).
    OMapScope
    suggested: None
    Statistical analysis: All patch clamp recorded data were analyzed using Clampfit 10.6 (Molecular Devices, USA), OriginPro 8.0 (Origin Lab, USA) and Adobe Illustrator 10 (Adobe, USA).
    OriginPro
    suggested: None
    Adobe Illustrator
    suggested: (Adobe Illustrator, RRID:SCR_010279)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.05.21.108605v3 on bioRxiv
  4. Version published to 10.1101/2020.05.21.108605v2 on bioRxiv
  5. Version published to 10.1101/2020.05.21.108605v1 on bioRxiv