Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 Replication by Hypertonic Saline Solution in Lung and Kidney Epithelial Cells
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SciScore for 10.1101/2020.08.04.235549: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources A clinical isolate SARS-CoV-2/SP02/human/2020/BRA (GenBank access n° MT126808.1) was propagated in Vero E6 cells, and viral titer was determined by 50% tissue culture infective dose (TCID50) and by plaque forming units per milliliter (PFU/mL), as previously described by Araujo and collaborators52. Vero E6suggested: NoneAntiviral activity of NaCl during different time-of-addition: Vero CCL-81 cells were seeded in a clear-bottomed … SciScore for 10.1101/2020.08.04.235549: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources A clinical isolate SARS-CoV-2/SP02/human/2020/BRA (GenBank access n° MT126808.1) was propagated in Vero E6 cells, and viral titer was determined by 50% tissue culture infective dose (TCID50) and by plaque forming units per milliliter (PFU/mL), as previously described by Araujo and collaborators52. Vero E6suggested: NoneAntiviral activity of NaCl during different time-of-addition: Vero CCL-81 cells were seeded in a clear-bottomed 96-well plate (5 × 104 cells/mL) and incubated for 24h at 37°C for cell adherence. Vero CCL-81suggested: NoneAnalysis of membrane potential variation by microfluorimetry: Changes of the membrane potential of Vero cells exposed to increasing concentrations of NaCl was determined by plate microfluorimetry recordings with the FlexStation III microplate reader and the FLIPR Membrane Potential Assay Kit (Molecular Devices Corp., Sunnyvale, CA) following the manufactures’ instructions, as previously described59. Verosuggested: NoneSoftware and Algorithms Sentences Resources Cell viability was further evaluated using a colorimetric assay by quantifying lactate dehydrogenase (LDH) released into the culture supernatant from cells with damaged membranes, using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega Corp., Madison, WI). CytoToxsuggested: NoneThe graphics were designed using GraphPad Prism 8.4.1 software. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT02438579 Completed Hypertonic Saline Nasal Irrigation and Gargling for the Comm… NCT04465604 Recruiting Hypertonic Saline for COVID-19 Symptoms NCT04382131 Recruiting Hypertonic Saline Nasal Irrigation and Gargling in Suspected… NCT04341688 Not yet recruiting A Clinical Trial of Gargling Agents in Reducing Intraoral Vi… Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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