Identification of Inhibitors of SARS-CoV-2 3CL-Pro Enzymatic Activity Using a Small Molecule in Vitro Repurposing Screen

Article activity feed

1. Version published to 10.1021/acsptsci.0c00216

   Mar 11, 2021
2. 

   ScreenIT

   Dec 17, 2020

   SciScore for 10.1101/2020.12.16.422677: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   After serial passaging on Huh7 and Vero-E6 cells, infectious content of the virus stock was determined by titration on Vero-E6 cells using the Spearman-Kärber method.	Huh7 suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336) Vero-E6 suggested: None
   The day before infection (Day −1), plates were equilibrated to room temperature and 30 μL of Vero-E6 EGFP cells were added to give 8,000 cells/well.	Vero-E6 EGFP suggested: None
   Briefly, VeroE6 …

   SciScore for 10.1101/2020.12.16.422677: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   After serial passaging on Huh7 and Vero-E6 cells, infectious content of the virus stock was determined by titration on Vero-E6 cells using the Spearman-Kärber method.	Huh7 suggested: CLS Cat# 300156/p7178_HuH7, RRID:CVCL_0336) Vero-E6 suggested: None
   The day before infection (Day −1), plates were equilibrated to room temperature and 30 μL of Vero-E6 EGFP cells were added to give 8,000 cells/well.	Vero-E6 EGFP suggested: None
   Briefly, VeroE6 cells were seeded in a 96-well cell culture plate in a density of 40,000 cells per well.	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Software and Algorithms
   Sentences	Resources
   SARS-CoV-2 strain BetaCov/Belgium/GHB-03021/2020 recovered from a nasopharyngeal swab taken from an asymptomatic patient returning from Wuhan, China in the beginning of February 2020 was sequenced on a MinION platform (Oxford Nanopore).	MinION suggested: (MinION, RRID:SCR_017985)
   Data analysis: Data analysis of assay development results was performed using GraphPad Prism 8.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: Thank you for sharing your data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.12.16.422677v1 on bioRxiv

   Dec 16, 2020