Development of a High-Throughput Homogeneous AlphaLISA Drug Screening Assay for the Detection of SARS-CoV-2 Nucleocapsid

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1. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.08.20.258129: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Reagents and Materials: The following item was purchased from ATCC: Vero-E6 (CRL-1586, RRID:CVCL_0574).	Vero-E6 detected: (IZSLER Cat# BS CL 87, RRID:CVCL_0574)
   Vero-E6 cell culture: Vero-E6 (grown in EMEM, 10% FBS, and 1% Penicillin/Streptomycin), were cultured in T175 flasks and passaged at 95% confluency.	Vero-E6 suggested: None
   Vero E6 cells were plated at 20,000 and 50,000 cells/well in 384-well plate (TC) and incubated overnight.	Vero E6

   SciScore for 10.1101/2020.08.20.258129: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Reagents and Materials: The following item was purchased from ATCC: Vero-E6 (CRL-1586, RRID:CVCL_0574).	Vero-E6 detected: (IZSLER Cat# BS CL 87, RRID:CVCL_0574)
   Vero-E6 cell culture: Vero-E6 (grown in EMEM, 10% FBS, and 1% Penicillin/Streptomycin), were cultured in T175 flasks and passaged at 95% confluency.	Vero-E6 suggested: None
   Vero E6 cells were plated at 20,000 and 50,000 cells/well in 384-well plate (TC) and incubated overnight.	Vero E6 suggested: RRID:CVCL_XD71)
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Vero-E6 cell culture: Vero-E6 (grown in EMEM, 10% FBS, and 1% Penicillin/Streptomycin), were cultured in T175 flasks and passaged at 95% confluency.	Vero-E6 suggested: None
   Software and Algorithms
   Sentences	Resources
   Statistical analysis and illustrations: Concentration-response curves were fit using non-linear regression, standard curve interpolation, and graphs were generated in Graphpad Prism V8.43.	Graphpad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
2. Version published to 10.1021/acsptsci.0c00122

   Oct 9, 2020
3. 

   Version published to 10.1101/2020.08.20.258129v1 on bioRxiv

   Aug 20, 2020