Identifying SARS-CoV-2 Entry Inhibitors through Drug Repurposing Screens of SARS-S and MERS-S Pseudotyped Particles

Version published to 10.1021/acsptsci.0c00112

Oct 19, 2020

SciScore for 10.1101/2020.07.10.197988: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement	not detected.
Randomization	not detected.
Blinding	not detected.
Power Analysis	not detected.
Sex as a biological variable	not detected.
Cell Line Authentication	not detected.

Table 2: Resources

Antibodies
Sentences	Resources
Mouse-anti-firefly luciferase antibody was purchased from Santa Cruz (sc-74548)	Mouse-anti-firefly luciferase antibody suggested: None Mouse-anti-firefly luciferase suggested: None
Permeabilization/blocking solution was removed, 1:1000 primary mouse-anti-luciferase antibody (Santa Cruz) was added, and incubated overnight at 4 °C.	mouse-anti-luciferase suggested: None
Secondary antibody (10 nm gold-conjugated Goat-α-Mouse IgG) was diluted 1:20 in filtered blocking …

SciScore for 10.1101/2020.07.10.197988: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement	not detected.
Randomization	not detected.
Blinding	not detected.
Power Analysis	not detected.
Sex as a biological variable	not detected.
Cell Line Authentication	not detected.

Table 2: Resources

Antibodies
Sentences	Resources
Mouse-anti-firefly luciferase antibody was purchased from Santa Cruz (sc-74548)	Mouse-anti-firefly luciferase antibody suggested: None Mouse-anti-firefly luciferase suggested: None
Permeabilization/blocking solution was removed, 1:1000 primary mouse-anti-luciferase antibody (Santa Cruz) was added, and incubated overnight at 4 °C.	mouse-anti-luciferase suggested: None
Secondary antibody (10 nm gold-conjugated Goat-α-Mouse IgG) was diluted 1:20 in filtered blocking solution.	Goat-α-Mouse IgG suggested: None
Experimental Models: Cell Lines
Sentences	Resources
Cell lines and cell culture: Vero E6 cells (ATCC #CRL-1586) were cultured in EMEM with 10% FBS.	Vero E6 suggested: None
Pseudotyped particle (PP) entry assays: 96-well format: Cells were seeded in 50 μL/well media (20,000 cells/well for Vero E6 and Huh7, and 40,000 cells/well for Calu-3 cells), and incubated at 37◻°C, 5% CO2 overnight (~16 h).	Huh7 suggested: None
384-well format: 10,000 cells/well of Calu-3 cells were seeded in 10 μL media, and incubated at 37□°C, 5% CO2 overnight (~16 h).	Calu-3 suggested: None
Experimental Models: Organisms/Strains
Sentences	Resources
Reagents: The following items were purchased from ThermoFisher: Dulbecco's Modified Eagle's Medium (DMEM) (11965092), Pen/Strep (15140), TrypLE (12604013), PBS -/- (w/o Ca2+ or Mg2+) (10010049), HCS Cell Mask Green (H32714)	PBS -/- suggested: None
Software and Algorithms
Sentences	Resources
Half-maximal efficacious concentration (EC50) and half-maximal cytotoxicity concentration (CC50) of compounds were calculated using Prism software (GraphPad Software, San Diego, CA).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
Average values, standard deviations, and data counts were generated using pivot tables in Microsoft Excel and data was plotted in Graphpad Prism.	Microsoft Excel suggested: (Microsoft Excel, RRID:SCR_016137) Graphpad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

Results from JetFighter: We did not find any issues relating to colormaps.

Results from rtransparent:

Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
No protocol registration statement was detected.

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Identifying SARS-CoV-2 Entry Inhibitors through Drug Repurposing Screens of SARS-S and MERS-S Pseudotyped Particles

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