Identifying SARS-CoV-2 Entry Inhibitors through Drug Repurposing Screens of SARS-S and MERS-S Pseudotyped Particles
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
-
SciScore for 10.1101/2020.07.10.197988: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Mouse-anti-firefly luciferase antibody was purchased from Santa Cruz (sc-74548) Mouse-anti-firefly luciferase antibodysuggested: NoneMouse-anti-firefly luciferasesuggested: NonePermeabilization/blocking solution was removed, 1:1000 primary mouse-anti-luciferase antibody (Santa Cruz) was added, and incubated overnight at 4 °C. mouse-anti-luciferasesuggested: NoneSecondary antibody (10 nm gold-conjugated Goat-α-Mouse IgG) was diluted 1:20 in filtered blocking … SciScore for 10.1101/2020.07.10.197988: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Mouse-anti-firefly luciferase antibody was purchased from Santa Cruz (sc-74548) Mouse-anti-firefly luciferase antibodysuggested: NoneMouse-anti-firefly luciferasesuggested: NonePermeabilization/blocking solution was removed, 1:1000 primary mouse-anti-luciferase antibody (Santa Cruz) was added, and incubated overnight at 4 °C. mouse-anti-luciferasesuggested: NoneSecondary antibody (10 nm gold-conjugated Goat-α-Mouse IgG) was diluted 1:20 in filtered blocking solution. Goat-α-Mouse IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cell lines and cell culture: Vero E6 cells (ATCC #CRL-1586) were cultured in EMEM with 10% FBS. Vero E6suggested: NonePseudotyped particle (PP) entry assays: 96-well format: Cells were seeded in 50 μL/well media (20,000 cells/well for Vero E6 and Huh7, and 40,000 cells/well for Calu-3 cells), and incubated at 37◻°C, 5% CO2 overnight (~16 h). Huh7suggested: None384-well format: 10,000 cells/well of Calu-3 cells were seeded in 10 μL media, and incubated at 37□°C, 5% CO2 overnight (~16 h). Calu-3suggested: NoneExperimental Models: Organisms/Strains Sentences Resources Reagents: The following items were purchased from ThermoFisher: Dulbecco's Modified Eagle's Medium (DMEM) (11965092), Pen/Strep (15140), TrypLE (12604013), PBS -/- (w/o Ca2+ or Mg2+) (10010049), HCS Cell Mask Green (H32714) PBS -/-suggested: NoneSoftware and Algorithms Sentences Resources Half-maximal efficacious concentration (EC50) and half-maximal cytotoxicity concentration (CC50) of compounds were calculated using Prism software (GraphPad Software, San Diego, CA). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Average values, standard deviations, and data counts were generated using pivot tables in Microsoft Excel and data was plotted in Graphpad Prism. Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Graphpad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-