Identifying SARS-CoV-2 Entry Inhibitors through Drug Repurposing Screens of SARS-S and MERS-S Pseudotyped Particles

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  1. SciScore for 10.1101/2020.07.10.197988: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Mouse-anti-firefly luciferase antibody was purchased from Santa Cruz (sc-74548)
    Mouse-anti-firefly luciferase antibody
    suggested: None
    Mouse-anti-firefly luciferase
    suggested: None
    Permeabilization/blocking solution was removed, 1:1000 primary mouse-anti-luciferase antibody (Santa Cruz) was added, and incubated overnight at 4 °C.
    suggested: None
    Secondary antibody (10 nm gold-conjugated Goat-α-Mouse IgG) was diluted 1:20 in filtered blocking solution.
    Goat-α-Mouse IgG
    suggested: None
    Experimental Models: Cell Lines
    Cell lines and cell culture: Vero E6 cells (ATCC #CRL-1586) were cultured in EMEM with 10% FBS.
    Vero E6
    suggested: None
    Pseudotyped particle (PP) entry assays: 96-well format: Cells were seeded in 50 μL/well media (20,000 cells/well for Vero E6 and Huh7, and 40,000 cells/well for Calu-3 cells), and incubated at 37◻°C, 5% CO2 overnight (~16 h).
    suggested: None
    384-well format: 10,000 cells/well of Calu-3 cells were seeded in 10 μL media, and incubated at 37□°C, 5% CO2 overnight (~16 h).
    suggested: None
    Experimental Models: Organisms/Strains
    Reagents: The following items were purchased from ThermoFisher: Dulbecco's Modified Eagle's Medium (DMEM) (11965092), Pen/Strep (15140), TrypLE (12604013), PBS -/- (w/o Ca2+ or Mg2+) (10010049), HCS Cell Mask Green (H32714)
    PBS -/-
    suggested: None
    Software and Algorithms
    Half-maximal efficacious concentration (EC50) and half-maximal cytotoxicity concentration (CC50) of compounds were calculated using Prism software (GraphPad Software, San Diego, CA).
    suggested: (PRISM, RRID:SCR_005375)
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Average values, standard deviations, and data counts were generated using pivot tables in Microsoft Excel and data was plotted in Graphpad Prism.
    Microsoft Excel
    suggested: (Microsoft Excel, RRID:SCR_016137)
    Graphpad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.