Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes

Article activity feed

1. Version published to 10.1021/acsomega.0c05635

   Mar 15, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.07.31.20165704: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The Sf9 cells were cultured in ESF 921 medium (Expression Systems) at 27°C with continuous shaking at 80 rpm for aeration.	Sf9 suggested: None
   Software and Algorithms
   Sentences	Resources
   The unit of His-Taq Pol’s activity was determined by interpolation from the standard curve using Prism8 software (GraphPad)	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   Then 1 µL of enzymes was added to the respective reaction of either MMLV-RT, …

   SciScore for 10.1101/2020.07.31.20165704: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   The Sf9 cells were cultured in ESF 921 medium (Expression Systems) at 27°C with continuous shaking at 80 rpm for aeration.	Sf9 suggested: None
   Software and Algorithms
   Sentences	Resources
   The unit of His-Taq Pol’s activity was determined by interpolation from the standard curve using Prism8 software (GraphPad)	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)
   Then 1 µL of enzymes was added to the respective reaction of either MMLV-RT, ProtoScript® II (New England Labs, Cat No M0368), NEB AMV (New England Labs, Cat No M0277), Superscript II (Invitrogen, Cat. No 18064022), or Superscript III (Invitrogen, Cat. No 18080093).	New England Labs suggested: None
   The one-step RT-qPCR was performed in ABI 7500 and 7900 Fast Real-Time PCR system (Applied Biosystems, USA) for the side-by-side comparison with the Invitrogen SuperScript™ III Platinum™ One-step RT-qPCR and Thermofisher TaqPath™ 1-Step RT-qPCR, respectively.	Thermofisher TaqPath™ suggested: None

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Read the original source
3. 

   Version published to 10.1101/2020.07.31.20165704v1 on medRxiv

   Aug 4, 2020