Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2020.08.12.247338: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend …
SciScore for 10.1101/2020.08.12.247338: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
SciScore for 10.1101/2020.08.12.247338: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Mice were cared for and treated in accordance with the National Institutes of Health (NIH) guidelines for the care and use of laboratory animals (NIH Publication No. 85e23 Rev. 1985) as approved by the Animal Experimental Ethics Committee of TMMU (AMUWEC2020799). Randomization not detected. Blinding not detected. Power Analysis The cracked membrane was obtained by centrifugation at 8,000 rpm for 10 min, washed with cold PBS containing protease inhibitors and sonicated with a Sonics (Newtown, CT, US) Vibra-Cell VCX-500 ultrasonic processor for 10 min at a power of 120 W. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibo… SciScore for 10.1101/2020.08.12.247338: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Mice were cared for and treated in accordance with the National Institutes of Health (NIH) guidelines for the care and use of laboratory animals (NIH Publication No. 85e23 Rev. 1985) as approved by the Animal Experimental Ethics Committee of TMMU (AMUWEC2020799). Randomization not detected. Blinding not detected. Power Analysis The cracked membrane was obtained by centrifugation at 8,000 rpm for 10 min, washed with cold PBS containing protease inhibitors and sonicated with a Sonics (Newtown, CT, US) Vibra-Cell VCX-500 ultrasonic processor for 10 min at a power of 120 W. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources A primary anti-ACE2 rabbit polyclonal antibody (1:1000, 10108-T26, Sino Biological, Beijing, CHN) and a goat anti-rabbit secondary antibody (1:1000, A0208, Beyotime) were employed to detect ACE2. anti-rabbitsuggested: (GenWay Biotech Inc. Cat# GWB-A0208E, RRID:AB_10270952)β-actin determined by a mouse monoclonal antibody (AA128, Beyotime, 1:1000) was a reference. AA128suggested: (Beyotime Cat# AA128, RRID:AB_2861213)A primary anti-His-tag mouse monoclonal antibody (1:1000, AF5060, Beyotime) and a goat anti-mouse secondary antibody (1:1000, A0216, Beyotime) were employed to detect SARS-CoV-2 S1. anti-His-tagsuggested: (AnaSpec; EGT Group Cat# 29673-1000, RRID:AB_11232932)anti-mousesuggested: (Beyotime Cat# A0216, RRID:AB_2860575)A primary anti-ACE2 rabbit polyclonal antibody (1:200, 10108-T60, Sino Biological) and a goat anti-rabbit secondary antibody (1:500, A0516, Beyotime) were employed to stain ACE2. ACE2suggested: NoneA primary anti-spike rabbit monoclonal antibody (1:100, 40150-R007, Sino Biological) and a goat anti-rabbit secondary antibody (Alexa Fluor 488, Invitrogen, Thermo Fisher Scientific) were used to stain S1. anti-spikesuggested: (Sino Biological Cat# 40150-R007, RRID:AB_2827979)Experimental Models: Cell Lines Sentences Resources Recombinant SARS-CoV-2 S1 (20 μg mL-1, 40591-V08H, Sino Biological) containing a His-tag preincubated with CMBNPs at 37 °C for 1 h were added to HK-2 cells and incubated for another 1 h. HK-2suggested: RRID:CVCL_YE28)Preparation of HEK-293T-hACE2 and HEK-293T NPs HEK-293T-hACE2 cells collected by trypsinization and centrifugation at 1500 rpm for 5 min were washed with cold sterile PBS and frozen at -80 °C and thawed at room temperature (repeated three times). HEK-293Tsuggested: NoneHEK-293T-hACE2suggested: NoneToxicological Evaluation HUVECs obtained from the cell bank of CAS were seeded in a 96-well plate at a density of 5 × 103 cells/well. HUVECssuggested: NoneSoftware and Algorithms Sentences Resources The MS/MS database searches were conducted using SEQUEST search algorithm through Proteome Discoverer platform (version 1.4, Thermo Fisher Scientific). Proteome Discoverersuggested: (Proteome Discoverer, RRID:SCR_014477)Gene names of encoded proteins identified in the proteomics analysis were uploaded into the online STRING database (Version 11.0) (https://string-db.org) for Gene Ontology (GO) annotation. STRINGsuggested: (STRING, RRID:SCR_005223)The experiment was conducted in triplicate and repeated twice, and the data were processed using FlowJo software (version 7.6.1). FlowJosuggested: (FlowJo, RRID:SCR_008520)Statistical Analysis The significant difference (P) between each group was calculated using SPSS 16.0 software and the LSD multiple-comparison test. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
-
