Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV-2

Article activity feed

1. Version published to 10.1021/acsinfecdis.0c00725

   Mar 16, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.07.31.20165019: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Ethical approval was granted from the Ethics Committee of University Hospital Tübingen; samples from 193 SARS-CoV-2 polymerase chain reaction (PCR) positive individuals (179/2020/BO2) and of 18 self-reported negative samples were collected (179/2020/BO2).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After washing in PBST, 30 µL of Phycoerythrin labelled anti-human IgG secondary antibody (diluted 1:200 in serum assay buffer; Dianova) was added and incubated for 45 min at 23 °C.	anti-human IgG suggested: None
   …

   SciScore for 10.1101/2020.07.31.20165019: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Ethical approval was granted from the Ethics Committee of University Hospital Tübingen; samples from 193 SARS-CoV-2 polymerase chain reaction (PCR) positive individuals (179/2020/BO2) and of 18 self-reported negative samples were collected (179/2020/BO2).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After washing in PBST, 30 µL of Phycoerythrin labelled anti-human IgG secondary antibody (diluted 1:200 in serum assay buffer; Dianova) was added and incubated for 45 min at 23 °C.	anti-human IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   SARS-CoV-2 virus lysate, the supernatant of infected human Caco-2 cells was purified.	Caco-2 suggested: None
   Software and Algorithms
   Sentences	Resources
   All statistical analyses were performed using GraphPad Prism 8 or R studio (version 1.3.959).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: Thank you for sharing your data.

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.07.31.20165019 on medRxiv

   Aug 4, 2020