Comparative Analysis of Primer–Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)
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SciScore for 10.1101/2020.02.25.964775: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources African green monkey kidney Vero cells (ATCC CCL-81) were infected with a clinical isolate SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020 provided from Korea CDC). Verosuggested: NoneSoftware and Algorithms Sentences Resources South Korea) and T7 terminator primer (5’ – GCTAGTTATTGCTCAGCGG – 3’, Macrogen) with AccuPower® PCR PreMix (-dye) kit (Bioneer Inc., South Korea). Macrogensuggested: (Macrogen, RRID:SCR_014454)PCR product was then used as in … SciScore for 10.1101/2020.02.25.964775: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources African green monkey kidney Vero cells (ATCC CCL-81) were infected with a clinical isolate SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020 provided from Korea CDC). Verosuggested: NoneSoftware and Algorithms Sentences Resources South Korea) and T7 terminator primer (5’ – GCTAGTTATTGCTCAGCGG – 3’, Macrogen) with AccuPower® PCR PreMix (-dye) kit (Bioneer Inc., South Korea). Macrogensuggested: (Macrogen, RRID:SCR_014454)PCR product was then used as in vitro transcription template using MEGAscript™ T7 Transcription Kit (Invitrogen Inc., CA, USA). MEGAscript™suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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