Comparative Analysis of Primer–Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)

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1. Version published to 10.1021/acsinfecdis.0c00464

   Aug 11, 2020
2. 

   ScreenIT

   Jun 4, 2020

   SciScore for 10.1101/2020.02.25.964775: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   African green monkey kidney Vero cells (ATCC CCL-81) were infected with a clinical isolate SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020 provided from Korea CDC).	Vero suggested: None
   Software and Algorithms
   Sentences	Resources
   South Korea) and T7 terminator primer (5’ – GCTAGTTATTGCTCAGCGG – 3’, Macrogen) with AccuPower® PCR PreMix (-dye) kit (Bioneer Inc., South Korea).	Macrogen suggested: (Macrogen, RRID:SCR_014454)
   PCR product was then used as in …

   SciScore for 10.1101/2020.02.25.964775: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   African green monkey kidney Vero cells (ATCC CCL-81) were infected with a clinical isolate SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020 provided from Korea CDC).	Vero suggested: None
   Software and Algorithms
   Sentences	Resources
   South Korea) and T7 terminator primer (5’ – GCTAGTTATTGCTCAGCGG – 3’, Macrogen) with AccuPower® PCR PreMix (-dye) kit (Bioneer Inc., South Korea).	Macrogen suggested: (Macrogen, RRID:SCR_014454)
   PCR product was then used as in vitro transcription template using MEGAscript™ T7 Transcription Kit (Invitrogen Inc., CA, USA).	MEGAscript™ suggested: None

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.02.25.964775v1 on bioRxiv

   Feb 27, 2020